Rab5a Antibody (HL1497) - Azide and BSA Free

Immunohistochemistry-Paraffin: Rab5a Antibody (HL1497) - Azide and BSA Free [NBP3-25618] - RAB5A antibody [HL1497] detects RAB5A protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. RAB5A stained by RAB5A antibody [HL1497] (NBP3-25618) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: Rab5a Antibody (HL1497) - Azide and BSA Free [NBP3-25618] - RAB5A antibody [HL1497] detects RAB5A protein by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. RAB5A stained by RAB5A antibody [HL1497] (NBP3-25618) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: Rab5a Antibody (HL1497) - Azide and BSA Free [NBP3-25618] - RAB5A antibody [HL1497] detects RAB5A protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded dog muscle. RAB5A stained by RAB5A antibody [HL1497] (NBP3-25618) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: Rab5a Antibody (HL1497) - Azide and BSA Free [NBP3-25618] - RAB5A antibody [HL1497] detects RAB5A protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded cat lymph node. RAB5A stained by RAB5A antibody [HL1497] (NBP3-25618) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: Rab5a Antibody (HL1497) - Azide and BSA Free [NBP3-25618] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB5A antibody [HL1497] (NBP3-25618) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Ca, Fe, Bv, RM
• Applications: WB, ICC/IF, IHC, IP
• Clone: HL1497
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Rab5a Antibody (HL1497) - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit Rab5a Antibody (HL1497) - Azide and BSA Free (NBP3-25618) is a recombinant monoclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Rab5a. The exact sequence is proprietary.
• Source: HEK293
• Predicted Species: Bovine (93%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: RAB5A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Assay dependent
  • Immunohistochemistry Assay dependent
  • Immunohistochemistry-Paraffin Assay dependent
  • Immunoprecipitation Assay dependent
  • Western Blot 1:500-1:3000
• Theoretical MW: 24 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Sheep (81%), Chicken (87%), Porcine (81%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for Rab5a Antibody (HL1497) - Azide and BSA Free

• RAB5A, member RAS oncogene family
• RAB5RAS-associated protein RAB5A
• ras-related protein Rab-5A

Background

Rab5 is a 24 kDa GTP-binding protein that regulates the fusion of plasma membrane -derived clathrin-coated vesicles with early endosomes , and homotypic fusion among early endosomes1. Localized to the cytoplasmic side of the plasma membrane, clathrin -coated vesicles and early endosomes, Rab5 appears to regulate vesicle fusion through a cycle of GDP/GTP e xchange and GTP hydrolysis. The different guanine nuc leotide binding states of Rab5 may affect its ability to associate or dissociate with membranes during endocytotic membrane traffic. The GTP-bound, or active, form of Rab5 associates with membrane s and regulates vesicle docking and fusion. Studies using a Rab5 mutant that hydrolyzed xanthosine 5 -triphosphate (XTP) indicated that nucleotide hydrolysis occurs even in the absence of membrane fusion. GTP hydrolysis by Rab5 may determine the frequency of membrane docking and fusion events.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RAB5A