R-Spondin 1 Antibody [Unconjugated]

• Reactivity: Hu
• Applications: B/N
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

R-Spondin 1 Antibody [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived human R-Spondin 1
  Arg31-Ala263
  Accession # Q2MKA7
• Specificity: Detects human R-Spondin 1 in direct ELISAs. In direct ELISAs, approximately 6% cross-reactivity with recombinant mouse R-Spondin 1 is observed and less than 1% cross-reactivity with recombinant human (rh) R-Spondin 2, rhR-Spondin 3, and rhR-Spondin 4 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: RSPO1
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 3-12 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for R-Spondin 1 Antibody [Unconjugated]

• Cristin 3
• CRISTIN3
• FLJ40906Roof plate-specific spondin-1
• HRspo1
• roof plate-specific spondin
• RSPO
• RSPO1
• RSpondin 1
• R-Spondin 1
• R-spondin homolog (Xenopus laevis)
• RSPONDIN
• R-spondin1
• R-spondin-1

Background

R-Spondin 1 (RSPO1, Roof plate-specific Spondin 1), also known as cysteine-rich and single thrombospondin domain containing protein 3 (Cristin 3), is a 27 kDa secreted protein that shares ~40% amino acid (aa) identity with three otherR-Spondin family members (1, 2). All R-Spondins regulate Wnt/ beta -catenin signaling, but have distinct expression patterns (1‑3). Like other R-Spondins, R-Spondin 1 contains two adjacent cysteine-rich furin-like domains (aa 34‑135) with one potential N‑glycosylation site, followed by a thrombospondin (TSP-1) motif (aa 147‑207) and a region rich in basic residues (aa 211‑263). Only the furin-like domains are needed for beta -catenin stabilization (2, 4). A putative nuclear localization signal at the C-terminus may allow some expression in the nucleus (5). Potential isoforms of 200 and 236 aa have an alternate, shorter N‑terminus or are missing aa 146‑208, respectively (6). Over aa 21‑263, human R-Spondin 1 shares 89%, 87%, 92%, 91%, 91% and 89% aa identity with mouse, rat, equine, canine, caprine and bovine R-Spondin 1, respectively. R-Spondin 1 is expressed in early development at the roof plate boundary and is thought to contribute to dorsal neural tube development (3, 5). In humans, rare disruptions of the R-Spondin 1 gene are associated with tendencies for XX sex reversal (phenotypic male) or hermaphroditism, indicating a role for R-Spondin 1 in gender-specific differentiation (7, 8). Disruption is also associated with palmoplantar keratosis (7, 8). Postnatally, R-Spondin 1 is expressed by neuroendocrine cells in the intestine, adrenal gland and pancreas, and by epithelia in kidney and prostate (9). Injection of recombinant R-Spondin 1 in mice causes activation of beta -catenin and proliferation of intestinal crypt epithelial cells, and ameliorates experimental colitis (9, 10). R-Spondin 1 regulates Wnt/ beta -catenin by competing with the Wnt antagonist DKK-1 for binding to the Wnt co-receptors, Kremen and LRP-6, reducing their DKK-1-mediated internalization (11). Reports differ on whether R-Spondin 1 binds LRP-6 directly (11‑13).

1. Chen, J-Z. et al. (2002) Mol. Biol. Rep. 29:287.
2. Kim, K.-A. et al. (2006) Cell Cycle 5:23.
3. Nam, J.-S. et al. (2007) Gene Expr. Patterns 7:306.
4. Kazanskaya, O. et al. (2004) Dev. Cell 7:525.
5. Kamata, T. et al. (2004) Biochim. Biophys. Acta 1676:51.
6. Entrez accession # EAX07331, ABA54598.
7. Tomaselli, S. et al. (2008) Hum. Mutat. 29:220.
8. Parma, P. et al. (2006) Nat. Genet. 38:1304.
9. Kim, K.-A. et al. (2005) Science 309:1256.
10. Zhao, J. et al. (2007) Gastroenterology 132:1331.
11. Binnerts, M.E. et al. (2007) Proc. Natl. Acad. Sci. USA 104:14700.
12. Nam, J.-S. et al. (2006) J. Biol. Chem. 281:13247.
13. Wei, Q. et al. (2007) J. Biol. Chem. 282:15903.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for R-Spondin 1 Antibody (AF4645)(1)

Publication using AF4645

• X Zhou, L Geng, D Wang, H Yi, G Talmon, J Wang R-Spondin1/LGR5 Activates TGF? Signaling and Suppresses Colon Cancer Metastasis Cancer Res., 2017-09-22;0(0):. 2017-09-22 [PMID: 28939678] (Neutralization, Human)

Bioinformatics

• Gene Symbol: RSPO1
• Uniprot:
  • Q2MKA7(Human)