QPRT Antibody (5D11)

Western Blot: QPRT Antibody (5D11) [H00023475-M01] - QPRT monoclonal antibody (M01), clone 5D11 Analysis of QPRT expression in HepG2.

Immunohistochemistry-Paraffin: QPRT Antibody (5D11) [H00023475-M01] - Analysis of monoclonal antibody to QPRT on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.

Sandwich ELISA: QPRT Antibody (5D11) [H00023475-M01] - Detection limit for recombinant GST tagged QPRT is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu, Av
• Applications: WB, ELISA, IHC
• Clone: 5D11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

QPRT Antibody (5D11) Summary

• Immunogen: QPRT (NP_055113, 198 a.a. ~ 297 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VEVECSSLQEAVQAAEAGADLVLLDNFKPEELHPTATVLKAQFPSVAVEASGGITLDNLPQFCGPHIDVISMGMLTQAAPALDFSLKLFAKEVAPVPKIH
• Specificity: QPRT - quinolinate phosphoribosyltransferase (nicotinate-nucleotide pyrophosphorylase (carboxylating))
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: QPRT
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Reactivity Notes

Human. Other species not tested. Avian reactivity reported in scientific literature (PMID: 23761975)

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for QPRT Antibody (5D11)

• EC 2.4.2.19
• nicotinate-nucleotide pyrophosphorylase (carboxylating)
• QAPRTase
• QPRTasenicotinate-nucleotide pyrophosphorylase [carboxylating]
• Quinolinate phosphoribosyltransferase [decarboxylating]
• quinolinate phosphoribosyltransferase

Background

This gene encodes a key enzyme in catabolism of quinolinate, an intermediate in the tryptophan-nicotinamide adenine dinucleotide pathway. Quinolinate acts as a most potent endogenous exitotoxin to neurons. Elevation of quinolinate levels in the brain has been linked to the pathogenesis of neurodegenerative disorders such as epilepsy, Alzheimer's disease, and Huntington's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for QPRT Antibody (H00023475-M01)(4)

Publications using H00023475-M01

• Gayathri S, Chai L, Bruce B et al. Kynurenine Pathway Modulation Reverses the Experimental Autoimmune Encephalomyelitis Mouse Disease Progression. J Neuroinflammation. 2020-06-06 [PMID: 32505212]
• Muniz-Lino MA, Rodriguez-Vazquez M, Chavez-Munguia B et al. Anti-apoptotic quinolinate phosphoribosyltransferase (QPRT) is a target gene of Wilms tumor gene 1 (WT1) protein in leukemic cells. Biochem Biophys Res Commun 2017-01-22 [PMID: 27889611]
• Lim CK, Yap MM, Kent SJ et al. Characterization of the kynurenine pathway and quinolinic Acid production in macaque macrophages. Int J Tryptophan Res. 2013-01-01 [PMID: 23761975] (Avian)
• Hinsch N, Frank M, Doring C et al. QPRT: a potential marker for follicular thyroid carcinoma including minimal invasive variant; a gene expression, RNA and immunohistochemical study. BMC Cancer. 26;9:93. 2009-03-26 [PMID: 19321014]

Bioinformatics

• Gene Symbol: QPRT
• Entrez:
  • 23475(Human)
• OMIM:
  • 606248(Human)
• Uniprot:
  • NP_055113(Human)