QKI/Quaking Antibody

Images

 
Western Blot: QKI/Quaking Antibody [NB100-41401] - (0.3ug/ml) staining of mouse brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

QKI/Quaking Antibody Summary

Immunogen
Peptide with sequence ETKEKPKPTPDY-C corresponding to N-Terminus according to NP_006766.1, NP_996735.1, NP_996736.1, NP_996737.1.
Specificity
This antibody is expected to recognise all reported isoforms (NP_006766.1; NP_996735.1; NP_996736.1; NP_996737.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
QKI
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • Peptide ELISA Detection limit 1:64000
Application Notes
WB: Approx. 38 kDa band observed in mouse brain lysates (calculated MW of 35.8 kDa band according to NP_068681.1).
Positive Control
QKI/Quaking Lysate (NBL1-15026)
Publications
Read Publication using NB100-41401.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Human, Rat, Canine, Porcine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for QKI/Quaking Antibody

  • DKFZp586I0923
  • HKQ
  • homolog of mouse quaking QKI (KH domain RNA binding protein)
  • Hqk
  • hqkI
  • protein quaking
  • QK
  • QK1
  • QK3
  • quaking homolog, KH domain RNA binding (mouse)
  • RNA binding protein HQK

Background

QKI belongs to a family of RNA-binding proteins that have an HNRNPK (MIM 600712) homology (KH) domain embedded in a 200-amino acid region called the GSG domain. Other members of this family include SAM68 (KHDRBS1; MIM 602489) and SF1 (MIM 601516) (Chen and Richard, 1998).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for QKI/Quaking Antibody (NB100-41401)(1)

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol QKI
Entrez
Uniprot