Pyruvate Dehydrogenase E2 Antibody

Western Blot: Pyruvate Dehydrogenase E2 Antibody [NBP2-20026] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 7. 5% SDS PAGE gel, diluted at 1:10000.

Immunocytochemistry/Immunofluorescence: Pyruvate Dehydrogenase E2 Antibody [NBP2-20026] - DLAT antibody detects DLAT protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: DLAT protein stained by DLAT antibody diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E2 Antibody [NBP2-20026] - Immunohistochemical analysis of paraffin-embedded Ovarian carcinoma, using antibody at 1:250 dilution.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Pyruvate Dehydrogenase E2 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Pyruvate Dehydrogenase E2. The exact sequence is proprietary.
• Localization: Mitochondrion matrix
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DLAT
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:5000-1:20000
• Theoretical MW: 69 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Pyruvate Dehydrogenase E2 Antibody

• 70 kDa mitochondrial autoantigen of primary biliary cirrhosis
• dihydrolipoamide S-acetyltransferase
• DLTA
• E2 component of pyruvate dehydrogenase complex
• EC 2.3.1
• M2 antigen complex 70 kDa subunit
• PBC
• PDC-E2EC 2.3.1.12
• PDCE2mitochondrial

Background

The DLAT gene encodes dihydrolipoamide acetyltransferase (EC 2.3.1.12), the E2 subunit of the mammalian pyruvate dehydrogenase complex (PDC; EC 1.2.4.1) of the inner mitochondrial membrane. Patients with primary biliary cirrhosis (PBC; MIM 109720) show autoantibodies to DLAT.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: DLAT
• Uniprot:
  • P10515(Human)