Pyruvate Dehydrogenase E2 Antibody

Images

 
Western Blot: Pyruvate Dehydrogenase E2 Antibody [NBP1-54795] - Reccomended Titration: 5.0 ug/ml Positive Control: Jurkat cell lysate
Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E2 Antibody [NBP1-54795] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X ...read more
Western Blot: Pyruvate Dehydrogenase E2 Antibody [NBP1-54795] - Sample Type : Huh7 lysate (50ug) Primary Antibody Dilution : 1:200 Image Submitted By : Partha Kasturi University of Kansas Medical Center
Western Blot: Pyruvate Dehydrogenase E2 Antibody [NBP1-54795] - Sample Tissue: Human Jurkat Antibody Dilution: 1.0 ug/ml

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Pyruvate Dehydrogenase E2 Antibody Summary

Immunogen
Synthetic peptides corresponding to DLAT(dihydrolipoamide S-acetyltransferase (E2 component of pyruvate dehydrogenase complex)) The peptide sequence was selected from the N terminal of DLAT. Peptide sequence WTPSSGATPRNRLLLQLLGSPGRRYYSLPPH
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DLAT
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
This is a rabbit polyclonal antibody against DLAT and was validated on Western Blot and immunohistochemistry-P

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 2% Sucrose
Preservative
0.09% Sodium Azide
Purity
Protein A purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for Pyruvate Dehydrogenase E2 Antibody

  • 70 kDa mitochondrial autoantigen of primary biliary cirrhosis
  • dihydrolipoamide S-acetyltransferase
  • DLTA
  • E2 component of pyruvate dehydrogenase complex
  • EC 2.3.1
  • M2 antigen complex 70 kDa subunit
  • PBC
  • PDC-E2EC 2.3.1.12
  • PDCE2mitochondrial

Background

DLAT is dihydrolipoamide acetyltransferase, the E2 subunit of the mammalian pyruvate dehydrogenase complex of the inner mitochondrial membrane. Patients with primary biliary cirrhosis show autoantibodies to DLAT.The DLAT gene encodes dihydrolipoamide acetyltransferase (EC 2.3.1.12), the E2 subunit of the mammalian pyruvate dehydrogenase complex (PDC; EC 1.2.4.1) of the inner mitochondrial membrane. Patients with primary biliary cirrhosis (PBC; MIM 109720) show autoantibodies to DLAT.[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol DLAT
Uniprot