Pyruvate Dehydrogenase E1 beta subunit Antibody

Western Blot: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-20024] - Sample (50 ug of whole cell lysate) A: Mouse Heart, 10% SDS PAGE gel, diluted at 1:10000.

Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-20024] - Pyruvate Dehydrogenase E1 beta subunit antibody detects Pyruvate Dehydrogenase E1 beta subunit protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Pyruvate Dehydrogenase E1 beta subunit antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-20024] - A. 50 ug rat heart lysate/extract 10 % SDS-PAGE Pyruvate Dehydrogenase E1 beta subunit antibody dilution: 1:5000.

Western Blot: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-20024] - Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 10% SDS PAGE gel, diluted at 1:1000.

Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-20024] - Pyruvate Dehydrogenase E1 beta subunit antibody detects Pyruvate Dehydrogenase E1 beta subunit protein at cytoplasm in mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Pyruvate Dehydrogenase E1 beta subunit antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Pyruvate Dehydrogenase E1 beta subunit antibody (NBP2-20024) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Pyruvate Dehydrogenase E1 beta subunit antibody detects Pyruvate Dehydrogenase E1 beta subunit protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Pyruvate Dehydrogenase E1 beta subunit protein stained by Pyruvate Dehydrogenase E1 beta subunit antibody (NBP2-20024) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 um.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Pyruvate Dehydrogenase E1 beta subunit Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Pyruvate Dehydrogenase E1 beta subunit. The exact sequence is proprietary.
• Localization: Mitochondrion matrix
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PDHB
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:10000
• Theoretical MW: 39 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Pyruvate Dehydrogenase E1 beta subunit Antibody

• DKFZp564K0164
• mitochondrial
• pyruvate dehydrogenase (lipoamide) beta
• pyruvate dehydrogenase, E1 beta polypeptide

Background

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Pyruvate Dehydrogenase E1 beta subunit Antibody (NBP2-20024)(1)

Publication using NBP2-20024

• Grimm M, Calgeer B, Teriete P et al. Targeting thiamine-dependent enzymes for metabolic therapies in oral squamous cell carcinoma? Clin Transl Oncol. 2015-07-16 [PMID: 26179749] (IHC-P, Human)

Bioinformatics

• Gene Symbol: PDHB
• Uniprot:
  • P11177(Human)