Pyruvate Dehydrogenase E1 beta subunit Antibodies
We offer Pyruvate Dehydrogenase E1 beta subunit Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot. Each Pyruvate Dehydrogenase E1 beta subunit Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Pyruvate Dehydrogenase E1 beta subunit Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the Pyruvate Dehydrogenase E1 beta subunit Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Pyruvate Dehydrogenase E1 beta subunit polyclonal antibodies and browse our Pyruvate Dehydrogenase E1 beta subunit monoclonal antibody catalog.
Alternate Names for Pyruvate Dehydrogenase E1 beta subunit Antibodies
anti-Pyruvate Dehydrogenase E1 beta subunit antibody, anti-PDHB antibody, anti-DKFZp564K0164 antibody, anti-mitochondrial antibody, anti-pyruvate dehydrogenase (lipoamide) beta antibody, anti-pyruvate dehydrogenase, E1 beta polypeptide antibody
7 Results for "pyruvate-dehydrogenase-e1-beta-subunit" in Primary Antibodies
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-38327] - Analysis in human heart muscle and pancreas tissues. Corresponding Pyruvate Dehydrogenase E1 beta subunit RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-beta-subunit-Antibody-Immunohistochemistry-Paraffin-NBP2-38327-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-38327] - Analysis in human heart muscle and pancreas tissues. Corresponding Pyruvate Dehydrogenase E1 beta subunit RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-38327] - Staining of human cerebral cortex, heart muscle, kidney and pancreas using Anti-Pyruvate Dehydrogenase E1 beta subunit antibody NBP2-38327 (A) shows similar protein distribution across tissues to independent antibody NBP1-87421 (B).](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-beta-subunit-Antibody-Immunohistochemistry-Paraffin-NBP2-38327-img0015.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-38327] - Analysis using Anti-PDHB antibody NBP2-38327 (A) shows similar pattern to independent antibody NBP1-87421 (B).](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-beta-subunit-Antibody-Western-Blot-NBP2-38327-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-38327] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-beta-subunit-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38327-img0004.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-38327] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in distal tubules.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-beta-subunit-Antibody-Immunohistochemistry-Paraffin-NBP2-38327-img0017.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-38327] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-beta-subunit-Antibody-Immunohistochemistry-Paraffin-NBP2-38327-img0006.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-38327] - Staining of human kidney.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-beta-subunit-Antibody-Immunohistochemistry-Paraffin-NBP2-38327-img0010.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-38327] - Staining of human cerebral cortex shows moderate positivity in neuropil.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-beta-subunit-Antibody-Immunohistochemistry-Paraffin-NBP2-38327-img0013.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-38327] - Staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-beta-subunit-Antibody-Immunohistochemistry-Paraffin-NBP2-38327-img0016.jpg)
![Western Blot: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-20024] - Sample (50 ug of whole cell lysate) A: Mouse Heart, 10% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-beta-subunit-Antibody-Western-Blot-NBP2-20024-img0003.jpg "Western Blot: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-20024] - Sample (50 ug of whole cell lysate) A: Mouse Heart, 10% SDS PAGE gel, diluted at 1:10000.")
![Immunocytochemistry/Immunofluorescence: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-20024] - Pyruvate Dehydrogenase E1 beta subunit antibody detects Pyruvate Dehydrogenase E1 beta subunit protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Pyruvate Dehydrogenase E1 beta subunit protein stained by Pyruvate Dehydrogenase E1 beta subunit antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-beta-subunit-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20024-img0006.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-20024] - Pyruvate Dehydrogenase E1 beta subunit antibody detects Pyruvate Dehydrogenase E1 beta subunit protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Pyruvate Dehydrogenase E1 beta subunit antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-beta-subunit-Antibody-Immunohistochemistry-Paraffin-NBP2-20024-img0007.jpg)
![Western Blot: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-20024] - A. 50 ug rat heart lysate/extract 10 % SDS-PAGE Pyruvate Dehydrogenase E1 beta subunit antibody dilution: 1:5000.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-beta-subunit-Antibody-Western-Blot-NBP2-20024-img0001.jpg)
![Western Blot: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-20024] - Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-beta-subunit-Antibody-Western-Blot-NBP2-20024-img0002.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-20024] - Immunohistochemical analysis of paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-beta-subunit-Antibody-Immunohistochemistry-Paraffin-NBP2-20024-img0004.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-20024] - Pyruvate Dehydrogenase E1 beta subunit antibody detects Pyruvate Dehydrogenase E1 beta subunit protein at cytoplasm in mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Pyruvate Dehydrogenase E1 beta subunit antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-beta-subunit-Antibody-Immunohistochemistry-Paraffin-NBP2-20024-img0005.jpg)
![Western Blot: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP1-87421] - Analysis using Anti-PDHB antibody NBP1-87421 (A) shows similar pattern to independent antibody NBP2-38327 (B).](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-beta-subunit-Antibody-Western-Blot-NBP1-87421-img0010.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP1-87421] - Analysis using Anti-PDHB antibody NBP1-87421 (A) shows similar pattern to independent antibody NBP2-38327 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP1-87421] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in distal tubules.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-beta-subunit-Antibody-Immunohistochemistry-Paraffin-NBP1-87421-img0017.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP1-87421] - Staining of human heart muscle shows high expression.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-beta-subunit-Antibody-Immunohistochemistry-Paraffin-NBP1-87421-img0006.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP1-87421] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-beta-subunit-Antibody-Immunohistochemistry-Paraffin-NBP1-87421-img0007.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP1-87421] - Staining in human heart muscle and pancreas tissues using anti-PDHB antibody. Corresponding PDHB RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-beta-subunit-Antibody-Immunohistochemistry-Paraffin-NBP1-87421-img0008.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP1-87421] - Staining of human kidney.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-beta-subunit-Antibody-Immunohistochemistry-Paraffin-NBP1-87421-img0011.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP1-87421] - Staining of human colon.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-beta-subunit-Antibody-Immunohistochemistry-Paraffin-NBP1-87421-img0012.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP1-87421] - Staining of human cerebral cortex shows moderate positivity in neuropil.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-beta-subunit-Antibody-Immunohistochemistry-Paraffin-NBP1-87421-img0013.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP1-87421] - Staining of human cerebral cortex, heart muscle, kidney and pancreas using Anti-Pyruvate Dehydrogenase E1 beta subunit antibody NBP1-87421 (A) shows similar protein distribution across tissues to independent antibody NBP2-38327 (B).](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-beta-subunit-Antibody-Immunohistochemistry-Paraffin-NBP1-87421-img0015.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP1-87421] - Staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-beta-subunit-Antibody-Immunohistochemistry-Paraffin-NBP1-87421-img0016.jpg)
![Western Blot: Pyruvate Dehydrogenase E1 beta subunit Antibody (7V8O5) [NBP3-16455] - Western blot analysis of extracts of various cell lines, using Pyruvate Dehydrogenase E1 beta subunit Rabbit mAb (NBP3-16455) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-beta-subunit-Antibody-7V8O5-Western-Blot-NBP3-16455-img0004.jpg "Western Blot: Pyruvate Dehydrogenase E1 beta subunit Antibody (7V8O5) [NBP3-16455] - Western blot analysis of extracts of various cell lines, using Pyruvate Dehydrogenase E1 beta subunit Rabbit mAb (NBP3-16455) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry: Pyruvate Dehydrogenase E1 beta subunit Antibody (7V8O5) [NBP3-16455] - Immunohistochemistry analysis of Pyruvate Dehydrogenase E1 beta subunit in paraffin-embedded mouse kidney tissue using Pyruvate Dehydrogenase E1 beta subunit Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16455_rabbit-pyruvate-dehydrogenase-e1-beta-subunit-mab-7v8o5-7220251244338.jpg)
![Immunohistochemistry: Pyruvate Dehydrogenase E1 beta subunit Antibody (7V8O5) [NBP3-16455] - Immunohistochemistry analysis of Pyruvate Dehydrogenase E1 beta subunit in paraffin-embedded human thyroid cancer tissue using Pyruvate Dehydrogenase E1 beta subunit Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16455_rabbit-pyruvate-dehydrogenase-e1-beta-subunit-mab-7v8o5-72202512443344.jpg)
![Immunohistochemistry: Pyruvate Dehydrogenase E1 beta subunit Antibody (7V8O5) [NBP3-16455] - Immunohistochemistry analysis of Pyruvate Dehydrogenase E1 beta subunit in paraffin-embedded human lung cancer tissue using Pyruvate Dehydrogenase E1 beta subunit Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16455_rabbit-pyruvate-dehydrogenase-e1-beta-subunit-mab-7v8o5-72202512432098.jpg)
![Immunohistochemistry: Pyruvate Dehydrogenase E1 beta subunit Antibody (7V8O5) [NBP3-16455] - Immunohistochemistry analysis of Pyruvate Dehydrogenase E1 beta subunit in paraffin-embedded human liver cancer tissue using Pyruvate Dehydrogenase E1 beta subunit Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16455_rabbit-pyruvate-dehydrogenase-e1-beta-subunit-mab-7v8o5-7220251243202.jpg)
![Immunohistochemistry: Pyruvate Dehydrogenase E1 beta subunit Antibody (7V8O5) [NBP3-16455] - Immunohistochemistry analysis of Pyruvate Dehydrogenase E1 beta subunit in paraffin-embedded human colon carcinoma tissue using Pyruvate Dehydrogenase E1 beta subunit Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16455_rabbit-pyruvate-dehydrogenase-e1-beta-subunit-mab-7v8o5-72202512435386.jpg)
![Immunohistochemistry: Pyruvate Dehydrogenase E1 beta subunit Antibody (7V8O5) [NBP3-16455] - Immunohistochemistry analysis of Pyruvate Dehydrogenase E1 beta subunit in paraffin-embedded rat kidney tissue using Pyruvate Dehydrogenase E1 beta subunit Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16455_rabbit-pyruvate-dehydrogenase-e1-beta-subunit-mab-7v8o5-72202512435399.jpg)
![Western Blot: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-20025] - Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-beta-subunit-Antibody-Western-Blot-NBP2-20025-img0001.jpg "Western Blot: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-20025] - Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 10% SDS PAGE gel, diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-20025] - Pyruvate Dehydrogenase E1 beta subunit Antibody detects Pyruvate Dehydrogenase E1 beta subunit protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Pyruvate Dehydrogenase E1 beta subunit protein stained by Pyruvate Dehydrogenase E1 beta subunit Antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-beta-subunit-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20025-img0004.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-20025] - Immunohistochemical analysis of paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-beta-subunit-Antibody-Immunohistochemistry-Paraffin-NBP2-20025-img0003.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-20025] - Immunohistochemical analysis of paraffin-embedded Testis, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-beta-subunit-Antibody-Immunohistochemistry-Paraffin-NBP2-20025-img0002.jpg)
![Western Blot: Pyruvate Dehydrogenase E1 beta subunit Antibody (2B2) [H00005162-M03] - Analysis of PDHB expression in transfected 293T cell line by PDHB monoclonal antibody (M03), clone 2B2. Lane 1: PDHB transfected lysatE (39.2 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-beta-subunit-Antibody-2B2-Western-Blot-H00005162-M03-img0007.jpg "Western Blot: Pyruvate Dehydrogenase E1 beta subunit Antibody (2B2) [H00005162-M03] - Analysis of PDHB expression in transfected 293T cell line by PDHB monoclonal antibody (M03), clone 2B2. Lane 1: PDHB transfected lysatE (39.2 KDa). Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody (2B2) [H00005162-M03] - Analysis of monoclonal antibody to PDHB on formalin-fixed paraffin-embedded human heart. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-beta-subunit-Antibody-2B2-Immunohistochemistry-Paraffin-H00005162-M03-img0005.jpg)
![Western Blot: Pyruvate Dehydrogenase E1 beta subunit Antibody (2B2) [H00005162-M03] - Analysis of PDHB expression in HeLa (Cat # L013V1).](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-beta-subunit-Antibody-2B2-Western-Blot-H00005162-M03-img0006.jpg)
![ELISA: Pyruvate Dehydrogenase E1 beta subunit Antibody (2B2) [H00005162-M03] - Detection limit for recombinant GST tagged PDHB is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-beta-subunit-Antibody-2B2-ELISA-H00005162-M03-img0003.jpg)
![Western Blot: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP3-38354] - Western blot analysis of various lysates using Pyruvate Dehydrogenase E1 beta subunit Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-38354_rabbit-pyruvate-dehydrogenase-e1-beta-subunit-pab-1012202411535810.jpg "Western Blot: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP3-38354] - Western blot analysis of various lysates using Pyruvate Dehydrogenase E1 beta subunit Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.")