Recombinant Human Pyridoxal Kinase/PDXK Protein

Product Discontinued

Recombinant Human Pyridoxal Kinase/PDXK Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids1-312 of Human Pyridoxal Kinase/PDXK
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMEEECR VLSIQSHVIR GYVGNRAATF PLQVLGFEID AVNSVQFSNH TGYAHWKGQV LNSDELQELY EGLRLNNMNK YDYVLTGYTR DKSFLAMVVD IVQELKQQNP RLVYVCDPVL GDKWDGEGSM YVPEDLLPVY KEKVVPLADI ITPNQFEAEL LSGRKIHSQE EALRVMDMLH SMGPDTVVIT SSDLPSPQGS NYLIVLGSQR RRNPAGSVVM ERIRMDIRKV DAVFVGTGDL FAAMLLAWTH KHPNNLKVAC EKTVSTLHHV LQRTIQCAKA QAGEGVRPSP MQLELRMVQS KRDIEDPEIV VQATVL

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: PDXK
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 37.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human Pyridoxal Kinase/PDXK Protein

• C21orf124
• C21orf97
• chromosome 21 open reading frame 124
• DKFZp566A071
• EC 2.7.1.35
• FLJ21324
• FLJ37311
• HEL-S-1a
• MGC15873
• MGC31754
• MGC52346
• PDXK
• PKH
• PKHchromosome 21 open reading frame 97
• PNK
• PNKhuman pyridoxal kinase, EC 2.7.1.3510FLJ31940
• PRED79
• pyridoxal (pyridoxine, vitamin B6) kinase
• Pyridoxal Kinase
• pyridoxamine kinase
• Pyridoxine kinase
• Vitamin B6 Kinase

Background

PDXK converts vitamin B6 to pyridoxal-5-phosphate (PLP), an essential cofactor in the intermediate metabolism of amino acids and neurotransmitters. The encoded protein is cytoplasmic and probably acts as a homodimer. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. Recombinant human PDXK protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: PDXK