Pygopus-2 Antibody - BSA Free

Immunohistochemistry-Paraffin: Pygopus-2 Antibody [NBP2-20019] - Pygopus-2 antibody detects Pygopus-2 protein at nucleus in rat intestine by immunohistochemical analysis. Sample: Paraffin-embedded rat intestine. Pygopus-2 antibody diluted at 1:500.

Western Blot: Pygopus-2 Antibody [NBP2-20019] - Pygopus-2 was immunoprecipitated from 5*107 cells of SW480 whole cell lysate using 1 ug of Pygopus-2 antibody. The precipitated Pygopus-2 was detected by Pygopus-2 Antibody diluted at 1:15000. A: IgG. B: Pygopus-2 antibody

Immunohistochemistry-Paraffin: Pygopus-2 Antibody [NBP2-20019] - Pygopus-2 antibody detects Pygopus-2 protein at nucleus in mouse stomach by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. Pygopus-2 antibody diluted at 1:500.

Immunoprecipitation: Pygopus 2 Antibody [NBP2-20019] - Pygopus 2 was immunoprecipitated from 5*10^7 cells of SW480 whole cell lysate using 1 ug of anti-pygopus 2 antibody. The precipitated pygopus 2 was detected by NBP2-20019 diluted at 1:15000. A: IP-IgG B: IP-pygopus 2 antibody.

Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Pygopus 2 antibody (NBP2-20019) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Pygopus 2 antibody detects Pygopus 2 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Pygopus 2 protein stained by Pygopus 2 antibody (NBP2-20019) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 um.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IP, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Pygopus-2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Pygopus-2 Antibody - BSA Free (NBP2-20019) is a polyclonal antibody validated for use in IHC, WB, ICC/IF, IP and ChIP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length mouse Pygopus-2 Recombinant protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PYGO2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
• Application Notes: ChIP assay- Assay dependent
• Theoretical MW: 38 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Pygopus-2 Antibody - BSA Free

• 1190004M21Rik
• FLJ33226
• PYGO2
• pygopus 2
• pygopus homolog 2 (Drosophila)
• pygopus homolog 2
• Pygopus2
• Pygopus-2

Background

ATPase which is responsible for recognizing, binding, unfolding and translocation of pupylated proteins into the bacterial 20S proteasome core particle. May be essential for opening the gate of the 20S proteasome via an interaction with its C-terminus, thereby allowing substrate entry and access to the site of proteolysis. Thus, the C-termini of the proteasomal ATPase may function like a 'key in a lock' to induce gate opening and therefore regulate proteolysis. Is required but not sufficient to confer resistance against the lethal effects of reactive nitrogen intermediates (RNI), antimicrobial molecules produced by activated macrophages and other cell types.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PYGO2