Pygopus-2 Antibody

Images

 
Independent Antibodies: Western Blot: Pygopus-2 Antibody [NBP1-46171] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. ...read more
Immunohistochemistry-Paraffin: Pygopus-2 Antibody [NBP1-46171] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Pygo2 used at a dilution of 1:200 (1ug/ml) Detection: DAB

Product Details

Summary
Reactivity Hu, Mu, ZeSpecies Glossary
Applications WB, ChIP, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Pygopus-2 Antibody Summary

Immunogen
The immunogen for this product maps to a region between residue 1 and 50 of human Pygopus 2 using the numbering given in entry NP_612157.1 (GeneID 90780).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PYGO2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Chromatin Immunoprecipitation 1:10-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
Application Notes
Use in Chromatin Immunoprecipitation was reported in a scientific publication (PMID: 23637336). Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Publications
Read Publications using
NBP1-46171 in the following applications:

  • 2 publications
  • WB
    1 publication

Reactivity Notes

Zebrafish reactivity reported in scientific literature (PMID: 30366904).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Pygopus-2 Antibody

  • 1190004M21Rik
  • FLJ33226
  • PYGO2
  • pygopus 2
  • pygopus homolog 2 (Drosophila)
  • pygopus homolog 2
  • Pygopus2
  • Pygopus-2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Pygopus-2 Antibody (NBP1-46171)(3)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar

FAQs for Pygopus-2 Antibody (NBP1-46171) (0)

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PYGO2
Entrez