Pygopus-2 Antibody (539730) [Alexa Fluor® 594]

• Reactivity: Hu
• Applications: WB, IHC, ICC/IF
• Clone: 539730
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 594

Pygopus-2 Antibody (539730) [Alexa Fluor® 594] Summary

• Immunogen: E. coli-derived recombinant human Pygopus-2
  Met160-Glu336
  Accession # NP_612157
• Specificity: Detects human Pygopus-2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Pygopus-1 or recombinant mouse Pygopus-1 is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Pygopus-2 Antibody (539730) [Alexa Fluor® 594]

• 1190004M21Rik
• FLJ33226
• PYGO2
• pygopus 2
• pygopus homolog 2 (Drosophila)
• pygopus homolog 2
• Pygopus2
• Pygopus-2

Background

Human Pygopus-2 (pygopus; the name of a legless Australian lizard) is a 41 kDa, 406 amino acid (aa) transcriptional coactivator.  It contains three domains: an N‑terminal NLS (aa 41-47), followed by a proline-rich region and a zinc-finger PHD-type domain (aa 330-387).  A potential internal alternate start site may generate an isoform that deletes the first 37 aa.  Pygopus 2 is a nuclear protein that acts in concert with BCL-9 and TCF to retain beta -Catenin in the nucleus during Wnt-signaling.  It does so by forming a “chain-of-adaptors” with the above three proteins.  Over the range of aa used for immunization, human pygopus 2 is 95% identical to mouse.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.