Pygopus-1 Antibody (466023) [Alexa Fluor® 647]

• Applications: WB
• Clone: 466023
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647

Pygopus-1 Antibody (466023) [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant human Pygopus‑1
  Ala2-Ala419
  Accession # Q9Y3Y4
• Specificity: Detects human and mouse Pygopus-1 in direct ELISAs and Western blots.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Human Pygopus-1 (PYGO1, the name of a legless Australian lizard) is a 45 kDa, 419 amino acid (aa) transcriptional coactivator. It contains four domains: an N‑terminal NLS (aa 35‑41), followed by a Pro-rich, Asn-rich, and zinc-finger PHD-type domain (aa 340‑398). It is a nuclear protein that acts in concert with BCL-9 to retain beta -catenin in the nucleus during Wnt-signaling. In particular, following beta -catenin activation and translocation to the nucleus, beta -catenin first binds to BCL-9 in a phosphorylation-independent manner. Its continued presence in the nucleus depends upon BCL-9 binding to Pygopus. Once anchored, beta -catenin interacts with TCF to activate Wnt-responsive genes. Human Pygopus-1 shares 87% aa sequence identity with mouse Pygopus-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.