Pygopus-1 Antibody (3E1) - Azide and BSA Free

Western Blot: Pygopus-1 Antibody (3E1) [H00026108-M13] - Analysis of PYGO1 expression in transfected 293T cell line by PYGO1 monoclonal antibody (M13), clone 3E1. Lane 1: PYGO1 transfected lysate (Predicted MW: 46.09 KDa). Lane 2: Non-transfected lysate.

Sandwich ELISA: Pygopus-1 Antibody (3E1) [H00026108-M13] - Detection limit for recombinant GST tagged PYGO1 is 0.1 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 3E1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Pygopus-1 Antibody (3E1) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Pygopus-1 Antibody (3E1) - Azide and BSA Free (H00026108-M13) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: PYGO1 (NP_056432, 101 a.a. ~ 200 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. YSTFRMPPHVPPRMSSPYCGPYSLRNQPHPFPQNPLGMGFNRPHAFNFGPHDNSSFGNPSYNNALSQNVNMPNQHFRQNPAENFSQIPPQNASQVSNPDL
• Specificity: PYGO1 - pygopus homolog 1 (Drosophila) (3E1)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PYGO1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Pygopus-1 Antibody (3E1) - Azide and BSA Free

• DKFZp547G0910
• Pygo1
• pygopus 1
• pygopus homolog 1 (Drosophila)
• pygopus homolog 1
• Pygopus1
• Pygopus-1
• pygopus-like protein 1

Background

PYGO1, also known as Pygopus homolog 1, is a 45kDa 419 amino acid protein. PYGO1 is involved in signal transduction through the Wnt pathway. Current research on PYGO1 is being conducted in relation to acute diarrhea and colorectal cancer. PYGO1 is linked to the WTN signaling pathway where it interacts with BCL9, LENG8, CTNNB1, SPTBN1 and GRB2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PYGO1
• Entrez:
  • 26108(Human)
• Uniprot:
  • NP_056432(Human)