Recombinant Human Purine Nucleoside Phosphorylase/PNP Protein

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SDS-Page: Purine Nucleoside Phosphorylase/PNP Recombinant Protein [NBP1-50872]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Func, PAGE
Concentration
1 mg/ml

Order Details

Recombinant Human Purine Nucleoside Phosphorylase/PNP Protein Summary

Description
A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-289 of Human Purine Nucleoside Phosphorylase/PNP

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MENGYTYEDY KNTAEWLLSH TKHRPQVAII CGSGLGGLTD KLTQAQIFDY GEIPNFPRST VPGHAGRLVF GFLNGRACVM MQGRFHMYEG YPLWKVTFPV RVFHLLGVDT LVVTNAAGGL NPKFEVGDIM LIRDHINLPG FSGQNPLRGP NDERFGDRFP AMSDAYDRTM RQRALSTWKQ MGEQRELQEG TYVMVAGPSF ETVAECRVLQ KLGADAVGMS TVPEVIVARH CGLRVFGFSL ITNKVIMDYE SLEKANHEEV LAAGKQAAQK LEQFVSILMA SIPLPDKAS

Details of Functionality
Specific activity is > 120 units/mg, and is defined as the amount of enzyme that phosphorolysis of 1.0 umole of inosine with inorganic phosphate per minute at pH 7.5 at 25C.
Source
E. coli
Protein/Peptide Type
Biologically Active Protein
Gene
PNP
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
34.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH8.0) containing 0.1M NaCl, 2mM DTT, 10% glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human Purine Nucleoside Phosphorylase/PNP Protein

  • EC 2.4.2.1
  • FLJ94043
  • FLJ97288
  • Inosine phosphorylase
  • MGC117396
  • MGC125915
  • MGC125916
  • NPFLJ97312
  • nucleoside phosphorylase
  • PNP
  • PRO1837
  • PUNP
  • purine nucleoside phosphorylase
  • purine-nucleoside:orthophosphate ribosyltransferase

Background

PNP belongs to the PNP/MTAP phosphorylase family of proteins. This protein catalyzes the reversible phosphorolysis of ribonucleosides and 2'-deoxyribonucleosides with specificity for guanine, hypoxanthine and their analogs. PNP deficiency is a rare autosomal recessive genetic disease associated with a severe defect in T-lymphocyte function and neurologic disorder in children, comprising four percent of combined immunodeficiency cases. Recombinant human PNP protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

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Bioinformatics

Gene Symbol PNP
Entrez