Purine Nucleoside Phosphorylase/PNP Overexpression Lysate

Images

 
Western Blot: Nucleoside phosphorylase Overexpression Lysate (Adult Normal) [NBL1-13737] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Nucleoside phosphorylase.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Purine Nucleoside Phosphorylase/PNP Overexpression Lysate Summary

Description

Purine Nucleoside Phosphorylase/PNP Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC210759

Accession#: NM_000270

Protein Tag: C-MYC/DDK
Gene
PNP

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
31.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Purine Nucleoside Phosphorylase/PNP Overexpression Lysate

  • EC 2.4.2.1
  • FLJ94043
  • FLJ97288
  • Inosine phosphorylase
  • MGC117396
  • MGC125915
  • MGC125916
  • NPFLJ97312
  • nucleoside phosphorylase
  • PNP
  • PRO1837
  • PUNP
  • purine nucleoside phosphorylase
  • purine-nucleoside:orthophosphate ribosyltransferase

Background

This gene encodes an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. The enzyme is trimeric, containing three identical subunits. Mutations which result in nucleoside phosphorylase deficiency result in defective T-cell (cell-mediated) immunity but can also affect B-cell immunity and antibody responses. Neurologic disorders may also be apparent in patients with immune defects. A known polymorphism at aa position 51 that does not affect enzyme activity has been described. A pseudogene has been identified on chromosome 2. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Purine Nucleoside Phosphorylase/PNP Lysate (NBL1-13737) (0)

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Bioinformatics

Gene Symbol PNP