PTTG1 Antibody (DCS-280)

Immunohistochemistry-Paraffin: Securin Antibody (DCS-280) [NB600-549] - Human tonsil stained with NB 600-549 using peroxidase-conjugate and AEC chromogen. Note nuclear staining of lympocytes in germinal center.

Product Discontinued

PTTG1 Antibody (DCS-280) Summary

• Immunogen: Human recombinant full length Pds1 protein
• Localization: Nuclear.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PTTG1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:100 - 200
  • Western Blot 1:100-1:2000
• Control:

  HeLa Whole Cell Lysate
  Human Tonsil Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4) and 0.2% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A purified

Alternate Names for PTTG1 Antibody (DCS-280)

• EAP1MGC126883
• ESP1-associated protein 1
• Esp1-associated protein
• hPTTG
• pituitary tumor-transforming 1
• Pituitary tumor-transforming gene 1 protein
• PTTGMGC138276
• securin
• Tumor-transforming protein 1
• TUTR1HPTTG

Background

Securin is a protein involved in anaphase trigerring. It has two identified roles, the first one is to help the transport of separase to the nucleus and the second role is to inhibit the catalytic activity of separase. Securin is ubiquitinated by the Anaphase Promoting Complex (APC), and then degraded by the Proteasome, releasing separase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PTTG1 Antibody (NB600-549)(3)

Publications using NB600-549

• Lean SC, Candia AA, Gulacsi E et al. Obesogenic diet in mice compromises maternal metabolic physiology and lactation ability leading to reductions in neonatal viability Acta physiologica (Oxford, England) 2022-07-26 [PMID: 35880402] (IHC-P, Human)
  
  Details:
  1:75 IHC dilution
• Hong, H;Joo, K;Park, SM;Seo, J;Kim, MH;Shin, E;Cheong, HI;Lee, JH;Kim, J; Extraciliary roles of the ciliopathy protein JBTS17 in mitosis and neurogenesis Ann. Neurol. 2019-04-20 [PMID: 31004438] (WB, Human)
• Shrishrimal S, Kosmacek E, Chatterjee A et al. The SOD Mimic, MnTE-2-PyP, Protects from Chronic Fibrosis and Inflammation in Irradiated Normal Pelvic Tissues Antioxidants. 2017-11-06 [PMID: 29113120] (WB, Human)

Bioinformatics

• Gene Symbol: PTTG1
• Entrez:
  • 3481(Human)