PTRH2 Antibody - BSA Free

Western Blot: PTRH2 Antibody [NBP3-12579] - Lane A: Jurkat Whole Cell LysateLane B: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:19 kDaObserved band size:19 kDa(We are unsure as to the identity of these extra bands.)

Immunohistochemistry-Paraffin: PTRH2 Antibody [NBP3-12579] - Staining of human PTRH2 in human liver with rabbit polyclonal antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: PTRH2 Antibody [NBP3-12579] - Staining of human PTRH2 in human kidney with rabbit polyclonal antibody at 1:500 dilution.

Immunoprecipitation: PTRH2 Antibody [NBP3-12579] - Lane A:0.5 mg Jurkat Whole Cell Lysate4 uL anti-PTRH2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PTRH2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 19 kDaObserved band size :19 kDa

Immunocytochemistry/Immunofluorescence: PTRH2 Antibody [NBP3-12579] - Staining of PTRH2 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-12579 antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PTRH2 Antibody - BSA Free Summary

• Description: This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
  
  Protein A & Antigen Affinity purified
• Immunogen: Produced in rabbits immunized with E. coli-derived Human PTRH2 fragment
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PTRH2
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Immunoprecipitation 1-5uL/mg of uLysate
  • Western Blot 1:1000-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for PTRH2 Antibody - BSA Free

• Bcl-2 inhibitor of transcription 1
• BIT1CGI-147
• EC 3.1.1.29
• mitochondrial
• peptidyl-tRNA hydrolase 2
• PTH2FLJ32471

Background

The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTRH2