PTRH2 Antibody - BSA Free

Western Blot: PTRH2 Antibody [NBP2-94026] - Analysis of extracts of various cell lines, using PTRH2 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.

Immunocytochemistry/Immunofluorescence: PTRH2 Antibody [NBP2-94026] - Analysis of MCF-7 cells using PTRH2 . Blue: DAPI for nuclear staining.

Immunohistochemistry-Paraffin: PTRH2 Antibody [NBP2-94026] - Paraffin-embedded rat brain using PTRH2 .

Immunohistochemistry-Paraffin: PTRH2 Antibody [NBP2-94026] - Paraffin-embedded mouse spinal cord using PTRH2 .

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PTRH2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PTRH2 Antibody - BSA Free (NBP2-94026) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 40-179 of human PTRH2 (NP_057161.1). LPKSKTSKTHTDTESEASILGDSGEYKMILVVRNDLKMGKGKVAAQCSHAAVSAYKQIQRRNPEMLKQWEYCGQPKVVVKAPDEETLIALLAHAKMLGLTVSLIQDAGRTQIAPGSQTVLGIGPGPADLIDKVTGHLKLY
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PTRH2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:100
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:2000
• Theoretical MW: 19 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for PTRH2 Antibody - BSA Free

• Bcl-2 inhibitor of transcription 1
• BIT1CGI-147
• EC 3.1.1.29
• mitochondrial
• peptidyl-tRNA hydrolase 2
• PTH2FLJ32471

Background

The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTRH2