PTRH2 Antibody - BSA Free

Western Blot: PTRH2 Antibody [NB100-55963] - Western blot analysis of Bit1 in Daudi cell lysate with Bit1 antibody using NB100-55963 at (A) 1, (B) 2, and (C) 4 ug/ml.

Immunocytochemistry/ Immunofluorescence: PTRH2 Antibody - BSA Free [NB100-55963] - Immunofluorescence of PTRH2 in Human Small Intestine cells with PTRH2 antibody at 20 ug/mL.

Immunohistochemistry: PTRH2 Antibody - BSA Free [NB100-55963] - Immunohistochemistry of PTRH2 in human small intestine tissue with PTRH2 antibody at 10 ug/mL.

• Reactivity: Hu, Mu, Bv
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

PTRH2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PTRH2 Antibody - BSA Free (NB100-55963) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 16 amino acid synthetic peptide from near the center of human PTRH2. The immunogen is located within amino acids 80 - 130 of PTRH2.
• Predicted Species: Bovine (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PTRH2
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 10 ug/mL
  • Immunohistochemistry-Paraffin
  • Western Blot 1-4 ug/mL
• Control:

  PTRH2 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for PTRH2 Antibody - BSA Free

• Bcl-2 inhibitor of transcription 1
• BIT1CGI-147
• EC 3.1.1.29
• mitochondrial
• peptidyl-tRNA hydrolase 2
• PTH2FLJ32471

Background

Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment. Bit1 (Bcl-2 inhibitor of transcription 1) was recently identified as being involved in this process. Bit1 is a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis where it forms a complex with AES, a small Groucho/transducin-like enhancer of split (TLE) protein and induces caspase-independent apoptosis. Both AES and TLE proteins are transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. It has been suggested that Bit1-AES complexes turn off a survival-promoting gene transcription program controlled by TLE. Interestingly, apoptosis of cells transfected with Bit1 and AES could be inhibited if the cells were allowed to attach to fibronectin through the alpha 5/ beta 1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha 5/ beta 1 integrin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTRH2
• Entrez:
  • 51651(Human)