PTP4A1 Antibody

Western Blot: PTP4A1 Antibody [NB100-93524] - (1ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PTP4A1 Antibody Summary

• Immunogen: Peptide with sequence KDSNGHRNNCCIQ corresponding to C-Terminus according to NP_003454.1.
• Epitope: KDSNGHRNNCCIQ
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PTP4A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:2000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 21 kDa band observed in A549 and HeLa lysates (calculated MW of 19.8 kDa band according to NP_003454.1).
• Control:

  PTP4A1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PTP4A1 Antibody

• EC 3.1.3.48
• HH72
• PRL1
• PRL-1
• PRL-1DKFZp779M0721
• Protein tyrosine phosphatase IVA1
• protein tyrosine phosphatase type IVA 1
• protein tyrosine phosphatase type IVA protein 1
• protein tyrosine phosphatase type IVA, member 1
• Protein-tyrosine phosphatase 4a1
• Protein-tyrosine phosphatase of regenerating liver 1
• PTP(CAAX1)
• PTP(CAAXI)
• PTPCAAX1PRL1PTP(CAAX1)

Background

The protein encoded by this gene belongs to a small class of prenylated protein tyrosine phosphatases (PTPs), which contains a PTP domain and a characteristic C-terminal prenylation motif. PTPs are cell signaling molecules that play regulatory roles in a variety of cellular processes. This tyrosine phosphatase is a nuclear protein, but may primarily associate with plasma membrane. The surface membrane association of this protein depends on its C-terminal prenylation. Overexpression of this gene in mammalian cells conferred a transformed phenotype, which implicated its role in the tumorigenesis. Studies in rat suggested that this gene may be an immediate-early gene in mitogen-stimulated cells. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PTP4A1 Antibody (NB100-93524)(1)

Publication using NB100-93524

• Peng Y, Genin A, Spinner NB et al. The gene encoding human nuclear protein tyrosine phosphatase, PRL-1. Cloning, chromosomal localization, and identification of an intron enhancer. J Biol Chem 1998-07-03 [PMID: 9642300]

Bioinformatics

• Gene Symbol: PTP4A1
• Entrez:
  • 7803(Human)
• Uniprot:
  • NP_003454.1(Human)