PTP4A1 Overexpression Lysate (Native)

Images

 
Western Blot: PTP4A1 Overexpression Lysate (Adult Normal) [NBL1-14959] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PTP4A1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

PTP4A1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC200435 and based on accession number NM_003463. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens protein tyrosine phosphatase type IVA, member 1 (PTP4A1), mRNA.
Gene
PTP4A1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
19.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for PTP4A1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for PTP4A1 Overexpression Lysate (Native)

  • EC 3.1.3.48
  • HH72
  • PRL1
  • PRL-1
  • PRL-1DKFZp779M0721
  • Protein tyrosine phosphatase IVA1
  • protein tyrosine phosphatase type IVA 1
  • protein tyrosine phosphatase type IVA protein 1
  • protein tyrosine phosphatase type IVA, member 1
  • Protein-tyrosine phosphatase 4a1
  • Protein-tyrosine phosphatase of regenerating liver 1
  • PTP(CAAX1)
  • PTP(CAAXI)
  • PTPCAAX1PRL1PTP(CAAX1)

Background

The protein encoded by this gene belongs to a small class of prenylated protein tyrosine phosphatases (PTPs), which contains a PTP domain and a characteristic C-terminal prenylation motif. PTPs are cell signaling molecules that play regulatory roles in a variety of cellular processes. This tyrosine phosphatase is a nuclear protein, but may primarily associate with plasma membrane. The surface membrane association of this protein depends on its C-terminal prenylation. Overexpression of this gene in mammalian cells conferred a transformed phenotype, which implicated its role in the tumorigenesis. Studies in rat suggested that this gene may be an immediate-early gene in mitogen-stimulated cells. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for PTP4A1 Lysate (NBL1-14959) (0)

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Bioinformatics

Gene Symbol PTP4A1
Entrez