PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free

Western Blot: PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free [NBP2-73729] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTPN1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTPN1.

Immunocytochemistry/Immunofluorescence: PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free [NBP2-73729] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PTPN1.

Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free [NBP2-73729] - Staining of paraffin-embedded Human lymphoma tissue using anti-PTPN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.

Flow Cytometry: PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free [NBP2-73729] - HEK293T cells transfected with either RC204902 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PTPN1 antibody and then analyzed.

Western Blot: PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free [NBP2-73729] - Analysis of extracts (35ug) from 9 different cell lines by using anti-PTPN1 monoclonal antibody.

Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free [NBP2-73729] - Staining of paraffin-embedded Human colon tissue within the normal limits using anti-PTPN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.

Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free [NBP2-73729] - Staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PTPN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.

Western Blot: PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free [NBP2-73729] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT) and PTPN1-Knockout Hela cells (KO) were separated by SDS-PAGE and immunoblotted with anti-PTPN1 monoclonal antibody. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody as a loading control.(1:500)F

• Reactivity: Hu, Rt, Ca, Pm, Pm
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready, KO
• Clone: OTI1D10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free Summary

• Description: Novus Biologicals Knockout (KO) Validated Mouse PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free (NBP2-01056) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human PTPN1(NP_002818) produced in HEK293 cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: PTPN1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Knockout Validated
  • Western Blot 1:500-2000
• Theoretical MW: 49.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for PTP1B/PTPN1 Antibody (OTI1D10) - Azide and BSA Free

• EC 3.1.3.48
• protein tyrosine phosphatase 1B
• protein tyrosine phosphatase, non-receptor type 1
• Protein-tyrosine phosphatase 1B
• PTP1B
• PTP-1B
• PTP1Bprotein tyrosine phosphatase, placental
• PTPN1
• tyrosine-protein phosphatase non-receptor type 1

Background

The non-transmembrane protein tyrosine phosphatase, PTP1B, comprises 435 amino acids, of which the C-terminal 114 residues have been implicated in controlling both localization and function of this enzyme. Increased phosphorylation of PTP1B is seen to accompany the transition from G2 to M phase of the cell cycle. Immunofluorescence experiments indicate that PTP-1B is localized predominantly in the endoplasmic reticulum (ER). Subcellular fractionation is consistent with this localization and establishes that PTP-1B is tightly associated with microsomal membranes, with its phosphatase domain oriented towards the cytoplasm. The finding of a tyrosine phosphatase on the ER suggests new possibilities for cellular events controlled by tyrosine phosphorylation (2). It has also been shown that PTP1B is a novel substrate for Akt and that phosphorylation of PTP1B by Akt at Ser(50) may negatively modulate its phosphatase activity creating a positive feedback mechanism for insulin signaling (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTPN1