PTP sigma/PTPRS Antibody [Unconjugated]

Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, CHP-100 human neuroblastoma cell line, and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human PTP sigma/PTPRS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3430) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human PTPR mu, PTPR kappa, LAR, PTPR sigma, PTPR delta, and PTPR beta (40 ng/lane) were included. A specific band for PTP sigma/PTPRS was detected at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

PTPs/PTPRS was detected in immersion fixed A431 human epithelial carcinoma cell line using Goat Anti-Human PTPs/PTPRS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3430) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

PTP sigma/PTPRS Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human PTP sigma /PTPRS
  Glu30-Gly1260
  Accession # NP_570924
• Specificity: Detects the endogenous cleaved extracellular domain of human PTP sigma /PTPRS in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human LAR, PTPR beta , PTPRδ, PTPRµ, or PTPR kappa is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PTPRS
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PTP sigma/PTPRS Antibody [Unconjugated]

• EC 3.1.3.48
• protein tyrosine phosphatase PTPsigma
• protein tyrosine phosphatase, receptor type, S
• protein tyrosine phosphatase, receptor type, sigma
• PTP sigma
• PTPR sigma
• PTPRS
• PTPSIGMA
• receptor-type tyrosine-protein phosphatase S
• Receptor-type tyrosine-protein phosphatase sigma
• R-PTP-S
• R-PTP-sigma

Background

Protein Tyrosine Phoshatase Receptor-type sigma (PTPR sigma ), also known as PTP sigma , is an integral membrane protein with a non-glycosylated molecular weight of 214 kilodaltons. The extracellular domain, which was used as immunogen, is cleaved near the cell membrane by an endoprotease to generate a glycated 140 kDa protein. Significant levels of this protein are found in the brain and in endocrine tissues such as the pituitary gland and pancreas. Knockout mice deficient in PTPR sigma have severe neurological deficits and most die shortly after birth.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PTP sigma/PTPRS Antibody (AF3430)(13)

Publications using AF3430

• Nakajima, C;Sawada, M;Umeda, E;Takagi, Y;Nakashima, N;Kuboyama, K;Kaneko, N;Yamamoto, S;Nakamura, H;Shimada, N;Nakamura, K;Matsuno, K;Uesugi, S;Vep?ek, NA;Küllmer, F;Nasufovi?, V;Uchiyama, H;Nakada, M;Otsuka, Y;Ito, Y;Herranz-Pérez, V;García-Verdugo, JM;Ohno, N;Arndt, HD;Trauner, D;Tabata, Y;Igarashi, M;Sawamoto, K; Identification of the growth cone as a probe and driver of neuronal migration in the injured brain Nature communications 2024-03-09 [PMID: 38461182] (Western Blot, Human)
• Avilés, EC;Wang, SK;Patel, S;Shi, S;Lin, L;Kefalov, VJ;Goodrich, LV;Cepko, CL;Xue, Y; High temporal frequency light response in mouse retina is mediated by ON and OFF bipolar cells and requires FAT3 signaling bioRxiv : the preprint server for biology 2023-11-04 [PMID: 37961274] (IHC, Mouse)
• N Ran, W Li, R Zhang, C Lin, J Zhang, Z Wei, Z Li, Z Yuan, M Wang, B Fan, W Shen, X Li, H Zhou, X Yao, X Kong, S Feng Autologous exosome facilitates load and target delivery of bioactive peptides to repair spinal cord injury Bioactive materials, 2022-07-22;25(0):766-782. 2022-07-22 [PMID: 37056263] (IHC, Mouse)
• N Apóstolo, SN Smukowski, J Vanderlind, G Condomitti, V Rybakin, J Ten Bos, L Trobiani, S Portegies, KM Vennekens, NV Gounko, D Comoletti, KD Wierda, JN Savas, J de Wit Synapse type-specific proteomic dissection identifies IgSF8 as a hippocampal CA3 microcircuit organizer Nat Commun, 2020-10-14;11(1):5171. 2020-10-14 [PMID: 33057002] (IHC, Western Blot, Mouse)
• MND Svensson, M Zoccheddu, S Yang, G Nygaard, C Secchi, KM Doody, K Slowikowsk, F Mizoguchi, F Humby, R Hands, E Santelli, C Sacchetti, K Wakabayash, DJ Wu, C Barback, R Ai, W Wang, GP Sims, P Mydel, T Kasama, DL Boyle, F Galimi, D Vera, ML Tremblay, S Raychaudhu, MB Brenner, GS Firestein, C Pitzalis, AH Ekwall, SM Stanford, N Bottini Synoviocyte-targeted therapy synergizes with TNF inhibition in arthritis reversal Sci Adv, 2020-06-26;6(26):eaba4353. 2020-06-26 [PMID: 32637608] (Western Blot, Mouse)
• H Munezane, H Oizumi, T Wakabayash, S Nishio, T Hirasawa, T Sato, A Harada, T Yoshida, T Eguchi, Y Yamanashi, T Hashimoto, T Iwatsubo Roles of Collagen XXV and Its Putative Receptors PTPsigma/delta in Intramuscular Motor Innervation and Congenital Cranial Dysinnervation Disorder Cell Rep, 2019-12-24;29(13):4362-4376.e6. 2019-12-24 [PMID: 31875546] (IHC, Mouse)
• Thomas B. Davis, Mingli Yang, Heiman Wang, Changgong Lee, Timothy J. Yeatman, W. Jack Pledger PTPRS drives adaptive resistance to MEK/ERK inhibitors through SRC Oncotarget 2019-11-26 [PMID: 31827720]
• Thomas B. Davis, Mingli Yang, Michael J. Schell, Heiman Wang, Le Ma, W. Jack Pledger et al. PTPRS Regulates Colorectal Cancer RAS Pathway Activity by Inactivating Erk and Preventing Its Nuclear Translocation Scientific Reports 2018-06-18 [PMID: 29915291]
• S Dyck, H Kataria, A Alizadeh, KT Santhosh, B Lang, J Silver, S Karimi-Abd Perturbing chondroitin sulfate proteoglycan signaling through LAR and PTP? receptors promotes a beneficial inflammatory response following spinal cord injury J Neuroinflammation, 2018-03-20;15(1):90. 2018-03-20 [PMID: 29558941] (ICC, Rat)
• Bunin A, Sisirak V, Ghosh H, Grajkowska L, Hou Z, Miron M, Yang C, Ceribelli M, Uetani N, Chaperot L, Plumas J, Hendriks W, Tremblay M, Hacker H, Staudt L, Green P, Bhagat G, Reizis B Protein Tyrosine Phosphatase PTPRS Is an Inhibitory Receptor on Human and Murine Plasmacytoid Dendritic Cells. Immunity, 2015-07-28;43(2):277-88. 2015-07-28 [PMID: 26231120] (Flow Cytometry, ICC, Human)
• Guillermo A. Gomez, Robert W. McLachlan, Selwin K. Wu, Benjamin J. Caldwell, Elliott Moussa, Suzie Verma et al. An RPTP alpha /Src family kinase/Rap1 signaling module recruits myosin IIB to support contractile tension at apical E-cadherin junctions Molecular Biology of the Cell 2015-04-01 [PMID: 25631816]
• Lang B, Cregg J, DePaul M, Tran A, Xu K, Dyck S, Madalena K, Brown B, Weng Y, Li S, Karimi-Abdolrezaee S, Busch S, Shen Y, Silver J Modulation of the proteoglycan receptor PTPsigma promotes recovery after spinal cord injury. Nature, 2014-12-03;518(7539):404-8. 2014-12-03 [PMID: 25470046] (ICC, Rat)
• A.R. Ketschek, C. Haas, G. Gallo, I. Fischer The roles of neuronal and glial precursors in overcoming chondroitin sulfate proteoglycan inhibition Experimental Neurology 2012-06-01 [PMID: 22498104]

Bioinformatics

• Gene Symbol: PTPRS
• Uniprot:
  • NP_570924()