PTEN Antibody

Product Discontinued

PTEN Antibody Summary

• Immunogen: Synthetic peptide corresponding to the residues surrounding Aspartate 386 of human PTEN
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PTEN
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analyzes of PTEN (D386) pAb in extracts from HT29 cells.
  Immunohistochemistry (IHC) analyzes of PTEN (D386) pAb in paraffin-embedded human lung carcinoma tissue.
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, Mouse, Rat

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (approx. pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PTEN Antibody

• BZS
• EC 3.1.3.16,10q23del
• EC 3.1.3.48
• EC 3.1.3.67
• GLM2
• MMAC1 phosphatase and tensin homolog deleted on chromosome 10
• MMAC1
• MMAC1MGC11227
• Mutated in multiple advanced cancers 1
• phosphatase and tensin homologDEC
• phosphatidylinositol-34,5-trisphosphate 3-phosphatase and dual-specificityprotein phosphatase PTEN
• PTEN
• PTEN1
• TEP1MHAM

Background

As human tumors progress to advanced stages, one genetic alteration that occurs at high frequency is a loss of heterozygosity (LOH) at chromosome 10q23. Mapping of homozygous deletions on this chromosome led to the isolation of the PTEN gene, also designated MMAC1 (for mutated in multiple advanced cancers) and TEP1. This candidate tumor suppressor gene exhibits a high frequency of mutations in human glioblastomas and is also mutated in other cancers, including sporadic brain, breast, kidney and prostate cancers. PTEN has been associated with Cowden disease, an autosomal dominant cancer predisposition syndrome. The PTEN gene product is a putative protein tyrosine phosphatase that is localized to the cytoplasm, and it shares extensive homology with the cytoskeletal proteins tensin and auxilin. Gene transfer studies have indicated that the phosphatase domain of PTEN is essential for growth suppression of glioma cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTEN
• Entrez:
  • 5728(Human)
  • 19211(Mouse)
  • 50557(Rat)