PTEN Antibody (28H6)

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Product Details

Summary
Product Discontinued
View other related PTEN Primary Antibodies

Order Details


    • Catalog Number
      NB200-175
    • Availability
      Product Discontinued

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PTEN Antibody (28H6) Summary

Immunogen
BALB/C mice were injected with prokaryotic recombinant protein corresponding to a 200 amino acid C-terminal region of the PTEN molecule.
Localization
Nuclear
Specificity
This antibody reacts with human PTEN also known as MMAC or TEP1. PTEN is a tumor suppressor gene, which encodes a multifunctional phosphatase and which is expressed almost ubiquitously and regulates the cell cycle, apoptosis, and cell adhesion. Deletions and mutations of PTEN occur in a range of cancers including breast cancer, malignant melanoma, endometrial carcinoma, bladder carcinoma, small cell lung carcinoma, and endometrial ovarian carcinoma.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PTEN
Purity
Tissue culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
This antibody is useful in Immunohistochemistry-Paraffin. This antibody may be diluted to a titer of 1:50-1:75 in an ABC method.
Publications
Read Publications using
NB200-175 in the following applications:

Reactivity Notes

Cross reacts with Human.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
No buffer
Preservative
Sodium Azide
Purity
Tissue culture supernatant

Alternate Names for PTEN Antibody (28H6)

  • BZS
  • EC 3.1.3.16,10q23del
  • EC 3.1.3.48
  • EC 3.1.3.67
  • GLM2
  • MMAC1 phosphatase and tensin homolog deleted on chromosome 10
  • MMAC1
  • MMAC1MGC11227
  • Mutated in multiple advanced cancers 1
  • phosphatase and tensin homologDEC
  • phosphatidylinositol-34,5-trisphosphate 3-phosphatase and dual-specificityprotein phosphatase PTEN
  • PTEN
  • PTEN1
  • TEP1MHAM

Background

The PTEN tumor suppressor gene acts as a phospholipid phosphatase. Under normal growth conditions, stimulatory signals from the insulin receptor activate the enzyme PI3-K, which phosphorylates phosphotidylinositol 4,5-bisphosphate (PIP2) to generate phosphotidylinositol 3,4,5-triphosphate (PIP3), a lipid molecule. Downstream, PIP3 activates several effectors, including the proto-oncogene product PKB/Akt. The role of PTEN is to dephosphorylate PIP3, acting as a negative control on PKB/Akt activation. If a mutation in PTEN causes it unable to carry out its phosphatase function, PIP3 can no longer be deactivated, so it continues to propagate its signal downstream. This may result in the continued activation of PKB/Akt, which, in combination with other factors, could lead to increased cell growth and possible tumor development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PTEN Antibody (NB200-175)(3)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for PTEN Antibody (NB200-175). (Showing 1 - 1 of 1 FAQs).

  1. I would like to know if these antibodies are suitable with IHC techniques using HRP conjugated antibodies and DAB as chromogen? Cat# NB200-175, NB100-904 and NB100-142
    • Yes, all of these antibodies are useful for IHC-P with DAB based detection. Please note that the PTEN Antibody (28H6) NB200-175 has been discontinued, however.

Secondary Antibodies

 

Isotype Controls

Additional PTEN Products

Bioinformatics Tool for PTEN Antibody (NB200-175)

Discover related pathways, diseases and genes to PTEN Antibody (NB200-175). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for PTEN Antibody (NB200-175)

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Research Areas for PTEN Antibody (NB200-175)

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Blogs on PTEN.

The effects of curcumin on IKB Alpha and the NFkB signaling pathway
The IKK complex, or inhibitor of NFkB kinase, is composed of IKK alpha and IKK beta.  These kinases are at the core of the NFkB signaling cascade.  The NFkB family is made up of transcription factors that are kept inactive in the cytoplasm through...  Read full blog post.

Carbonic anhydrase IX (CAIX) - a reliable histochemical marker of hypoxia
Carbonic anhydrase IX is a member of the carbonic anhydrase family. This family consists of catalytic enzymes capable of converting carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. This family of molecules is abundantly e...  Read full blog post.

CAIX - One of the Best Cellular Markers of Hypoxia
The protein, carbonic anhydrase IX, belongs to the carbonic anhydrase family which consists of enzymes that rapidly convert carbon dioxide and water into the end products of carbonic acid, protons, and bicarbonate ions. These enzymes play a widespread...  Read full blog post.

Getting SHIP-shape Over Tumour Suppression
PTEN antibodies have shown PTEN to be an important tumor suppressor and, in mutated form, a factor in cancer development. However, a recent study, led by Robert Rickert, shows that the SHIP gene may also be an important tumor suppressor in B-cell lymp...  Read full blog post.

PTEN Antibodies and Cancer Research
Phosphatase and tensin homologue (PTEN) antibodies are important tools for cancer research. PTEN is an important tumor suppressor but, in mutated form, is also expressed in a high number of cancers. We at Novus Biologicals have a wide PTEN antibody da...  Read full blog post.

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Bioinformatics

Gene Symbol PTEN
Entrez