PTEN Antibody (28H6)



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PTEN Antibody (28H6) Summary

BALB/C mice were injected with prokaryotic recombinant protein corresponding to a 200 amino acid C-terminal region of the PTEN molecule.
This antibody reacts with human PTEN also known as MMAC or TEP1. PTEN is a tumor suppressor gene, which encodes a multifunctional phosphatase and which is expressed almost ubiquitously and regulates the cell cycle, apoptosis, and cell adhesion. Deletions and mutations of PTEN occur in a range of cancers including breast cancer, malignant melanoma, endometrial carcinoma, bladder carcinoma, small cell lung carcinoma, and endometrial ovarian carcinoma.
IgG1 Kappa
Tissue culture supernatant
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Sodium Azide
Tissue culture supernatant


Application Notes
This antibody is useful in Immunohistochemistry-Paraffin. This antibody may be diluted to a titer of 1:50-1:75 in an ABC method.

Reactivity Notes

Cross reacts with Human.


Staining Protocol: We suggest an incubation period of 30 to 60 minutes at room temperature. Optimal incubation conditions should be determined by the user based upon the fixation conditions and staining system employed. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.


The PTEN tumor suppressor gene acts as a phospholipid phosphatase. Under normal growth conditions, stimulatory signals from the insulin receptor activate the enzyme PI3-K, which phosphorylates phosphotidylinositol 4,5-bisphosphate (PIP2) to generate phosphotidylinositol 3,4,5-triphosphate (PIP3), a lipid molecule. Downstream, PIP3 activates several effectors, including the proto-oncogene product PKB/Akt. The role of PTEN is to dephosphorylate PIP3, acting as a negative control on PKB/Akt activation. If a mutation in PTEN causes it unable to carry out its phosphatase function, PIP3 can no longer be deactivated, so it continues to propagate its signal downstream. This may result in the continued activation of PKB/Akt, which, in combination with other factors, could lead to increased cell growth and possible tumor development.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Carbonic anhydrase IX (CAIX) - a reliable histochemical marker of hypoxia
Carbonic anhydrase IX is a member of the carbonic anhydrase family. This family consists of catalytic enzymes capable of converting carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. This family of molecules is abundantly e...  Read full blog post.

CAIX - One of the Best Cellular Markers of Hypoxia
The protein, carbonic anhydrase IX, belongs to the carbonic anhydrase family which consists of enzymes that rapidly convert carbon dioxide and water into the end products of carbonic acid, protons, and bicarbonate ions. These enzymes play a widespread...  Read full blog post.

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PTEN antibodies have shown PTEN to be an important tumor suppressor and, in mutated form, a factor in cancer development. However, a recent study, led by Robert Rickert, shows that the SHIP gene may also be an important tumor suppressor in B-cell lymp...  Read full blog post.

PTEN Antibodies and Cancer Research
Phosphatase and tensin homologue (PTEN) antibodies are important tools for cancer research. PTEN is an important tumor suppressor but, in mutated form, is also expressed in a high number of cancers. We at Novus Biologicals have a wide PTEN antibody da...  Read full blog post.

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Product PDFs


Gene Symbol PTEN

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