PSGL-1/CD162 Antibody (PSGL1/1567)

• Reactivity: Hu
• Applications: Flow, ICC/IF
• Clone: PSGL1/1567
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

PSGL-1/CD162 Antibody (PSGL1/1567) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Column Chromatography. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08695)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Recombinant human PSGL-1/CD162 protein (Uniprot: Q14242)
• Localization: Cell Surface
• Isotype: IgM Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SELPLG
• Purity: Column Chromatography

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
• Application Notes: Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Column Chromatography

Alternate Names for PSGL-1/CD162 Antibody (PSGL1/1567)

• CD162 antigen
• CD162
• P-selectin glycoprotein ligand 1
• PSGL1
• PSGL-1
• PSGL-1cutaneous lymphocyte-associated associated antigen
• selectin P ligandCLA
• SELPLG

Background

CD162, also known as p-selectin glycoprotein ligand-1 (PSGL-1), is a 120 - 220 kDa, mucin-like type I transmembrane glycoprotein. CD162 binds to CD62P (P-Selectin), CD62E (E-Selectin) and CD62L (L-Selectin). The interactions between P-selectin and P-selectin glycoprotein ligand-1 (PSGL-1) mediate the earliest "rolling" of leukocytes on the lumenal surface of activated endothelium, and the interaction between leukocytes and activated platelets or other leukocytes found at sites of inflammation. CD162 is expressed on neutrophils, monocytes, and most lymphocytes including NK and T cells but PSGL-1 stains B cells at significantly lower levels than other cell types.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SELPLG
• Uniprot:
  • Q14242()
  • Q14242()