PSENEN/PEN2 Antibody

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Western Blot: PEN2 Antibody [NB300-227] - Analysis of PEN2 on human placenta.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

PSENEN/PEN2 Antibody Summary

Immunogen
A synthetic peptide corresponding to the C-terminus of human PEN2 (within residues 50-101). [UniProt# Q9NZ42]
Localization
Membrane
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PSENEN
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoprecipitation 1:50-1:100
  • Western Blot 1:1000-1:2500
Application Notes
This PEN2 antibody is useful for Immunoprecipitation and Western blot, where a band can be seen at approximately 12 kDa. This antibody has primarily been tested in HA-PEN2 transfected cells combining Immunoprecipitation with Western blot analysis (ECL). For endogenous sample testing, it is suggested that the investigator load a greater amount of protein on the gel and to combine Immunoprecipitation with Western blot analysis.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
12 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
PSENEN/PEN2 Overexpression Lysate
Publications
Read Publication using
NB300-227 in the following applications:

  • IP
    1 publication
  • WB
    1 publication

Reactivity Notes

Human and mouse neuroblastoma (N2a) cells.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for PSENEN/PEN2 Antibody

  • gamma-secretase subunit PEN-2
  • MSTP064
  • PEN-2
  • PEN2hematopoietic stem/progenitor cells protein MDS033
  • presenilin enhancer 2 homolog (C. elegans)
  • Presenilin enhancer protein 2
  • PSENEN

Background

PEN2 (gamma-secretase subunit PEN-2 or presenilin enhancer protein 2) is an essential subunit of the gamma-secretase complex which is an endoprotease complex responsible for catalyzing the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Gamma-secretase complex is composed of a presenilin homodimer (PSEN1 or PSEN2), nicastrin (NCSTN), APH1 (APH1A or APH1B) and PSENEN/PEN2. PEN2 represents the last step of maturation of gamma-secretase, facilitating endoproteolysis of presenilin and conferring gamma-secretase activity. PEN2 is a multi-pass membrane protein predominantly localized in the endoplasmic reticulum and in the Golgi stack membrane/cis-Golgi. Defects in PEN2 have been linked to familial acne inversa type 2 (ACNINV2), a chronic relapsing inflammatory disease of the hair follicles which is primarily characterized by recurrent draining sinuses, painful skin abscesses, and disfiguring scars.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PSENEN/PEN2 Antibody (NB300-227)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: IP, WB.


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(1)
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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PSENEN
Entrez
Uniprot