NFIB Antibody

Images

 
Western Blot: NFIB Antibody [NBP1-81000] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: NFIB Antibody [NBP1-81000] - Staining of human cell line U-251 MG shows localization to nucleoplasm & nucleoli fibrillar center. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: NFIB Antibody [NBP1-81000] - Staining of human skin shows moderate nuclear positivity in squamous epithelial cells.
Western Blot: NFIB Antibody [NBP1-81000] - Analysis in human cell line SH-SY5Y.
Immunohistochemistry-Paraffin: NFIB Antibody [NBP1-81000] - Staining of human cerebral cortex shows moderate nuclear positivity in neurons.
Immunohistochemistry-Paraffin: NFIB Antibody [NBP1-81000] - Staining of human pancreas shows moderate nuclear positivity in exocrine glandular cells.
Immunohistochemistry-Paraffin: NFIB Antibody [NBP1-81000] - Staining of human prostate shows moderate nuclear positivity in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ChIP, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

NFIB Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: VKSGVFNVSELVRVSRTPITQGTGVNFPIGEIPSQPYYHDMNSGVNLQRSLSSPPSSKRPKTISIDENMEPSPTGDFYPSPSSPAAGSRTWHERDQDMSSPTTMKKPEKPLFSSASPQDSSPRLSTFPQHHHPGIPGVAHSVISTRTP
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NFIB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
ICC/IF reported in scientific literature (PMID: 25063196). Chip reported in scientific literature (PMID: 24801505). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: PFA/Triton X-100
Control Peptide
NFIB Protein (NBP1-81000PEP)
Publications
Read Publications using
NBP1-81000 in the following applications:

  • 1 publication
  • 1 publication
  • IHC
    2 publications
  • WB
    1 publication

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24553933).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for NFIB Antibody

  • CCAAT-box-binding transcription factor
  • CTF
  • HMGIC/NFIB
  • NF-I/B
  • NFI-B
  • NFIB2
  • NFIB3
  • NFI-RED
  • nuclear factor 1 B-type
  • Nuclear factor 1/B
  • nuclear factor I/BNF1-B
  • TGGCA-binding protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NFIB Antibody (NBP1-81000)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ChIP, ICC/IF, IHC, WB.


Filter By Application
ChIP
(1)
ICC/IF
(1)
IHC
(2)
WB
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(3)
All Species

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for NFIB Antibody (NBP1-81000) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NFIB
Entrez
Uniprot