NFIB Antibody

Images

 
Western Blot: NFIB Antibody [NBP1-81000] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: NFIB Antibody [NBP1-81000] - Staining of human cell line U-251 MG shows localization to nucleoplasm & nucleoli fibrillar center. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: NFIB Antibody [NBP1-81000] - Staining of human heart muscle shows strong nuclear positivity in myocytes.
Western Blot: NFIB Antibody [NBP1-81000] - Analysis in human cell line SH-SY5Y.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

NFIB Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: VKSGVFNVSELVRVSRTPITQGTGVNFPIGEIPSQPYYHDMNSGVNLQRSLSSPPSSKRPKTISIDENMEPSPTGDFYPSPSSPAAGSRTWHERDQDMSSPTTMKKPEKPLFSSASPQDSSPRLSTFPQHHHPGIPGVAHSVISTRTP
Specificity
Specificity of human, mouse, rat NFIB antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NFIB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: PFA/Triton X-100
Control Peptide
NFIB Protein (NBP1-81000PEP)
Publications
Read Publications using
NBP1-81000 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24553933).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for NFIB Antibody

  • CCAAT-box-binding transcription factor
  • CTF
  • HMGIC/NFIB
  • NF-I/B
  • NFI-B
  • NFIB2
  • NFIB3
  • NFI-RED
  • nuclear factor 1 B-type
  • Nuclear factor 1/B
  • nuclear factor I/BNF1-B
  • TGGCA-binding protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NFIB Antibody (NBP1-81000)(4)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional Array Products

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Diseases for NFIB Antibody (NBP1-81000)

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Blogs on NFIB

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Calculators

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Bioinformatics

Gene Symbol NFIB