NFIB Antibody

Images

 
Western Blot: NFIB Antibody [NBP1-81000] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: NFIB Antibody [NBP1-81000] - Staining of human cell line U-251 MG shows localization to nucleoplasm & nucleoli fibrillar center. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: NFIB Antibody [NBP1-81000] - Staining of human skin shows moderate nuclear positivity in squamous epithelial cells.
Western Blot: NFIB Antibody [NBP1-81000] - Analysis in human cell line SH-SY5Y.
Immunohistochemistry-Paraffin: NFIB Antibody [NBP1-81000] - Staining of human cerebral cortex shows moderate nuclear positivity in neurons.
Immunohistochemistry-Paraffin: NFIB Antibody [NBP1-81000] - Staining of human pancreas shows moderate nuclear positivity in exocrine glandular cells.
Immunohistochemistry-Paraffin: NFIB Antibody [NBP1-81000] - Staining of human prostate shows moderate nuclear positivity in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, ChIP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

NFIB Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: VKSGVFNVSELVRVSRTPITQGTGVNFPIGEIPSQPYYHDMNSGVNLQRSLSSPPSSKRPKTISIDENMEPSPTGDFYPSPSSPAAGSRTWHERDQDMSSPTTMKKPEKPLFSSASPQDSSPRLSTFPQHHHPGIPGVAHSVISTRTP
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NFIB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
ICC/IF reported in scientific literature (PMID: 25063196). Chip reported in scientific literature (PMID: 24801505). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: PFA/Triton X-100
Control Peptide
NFIB Protein (NBP1-81000PEP)
Publications
Read Publications using
NBP1-81000 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24553933).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for NFIB Antibody

  • CCAAT-box-binding transcription factor
  • CTF
  • HMGIC/NFIB
  • NF-I/B
  • NFI-B
  • NFIB2
  • NFIB3
  • NFI-RED
  • nuclear factor 1 B-type
  • Nuclear factor 1/B
  • nuclear factor I/BNF1-B
  • TGGCA-binding protein

Background

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NFIB Antibody (NBP1-81000)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: Chemotaxis, ICC/IF, IF/IHC, WB.


Filter By Application
Chemotaxis
(1)
ICC/IF
(1)
IF/IHC
(2)
WB
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(3)
All Species

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NFIB Antibody (NBP1-81000) (0)

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Bioinformatics

Gene Symbol NFIB
Entrez
Uniprot