PSD-95 Antibody (7E3) - BSA Free

Western Blot: PSD-95 Antibody (7E3) [NBP2-12872] - Western Blot analysis of Rat brain membrane lysate showing detection of PSD-95 protein using Mouse Anti-PSD-95 Monoclonal Antibody, Clone 7E3 (NBP2-12872). Primary Antibody: Mouse Anti-PSD-95 Monoclonal Antibody (NBP2-12872) at 1:1000.

Immunocytochemistry/Immunofluorescence: PSD-95 Antibody (7E3) [NBP2-12872] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PSD-95 Monoclonal Antibody, Clone 7E3 (NBP2-12872). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-PSD-95 Monoclonal Antibody (NBP2-12872) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Filamentous-like staining.

Immunohistochemistry: PSD-95 Antibody (7E3) [NBP2-12872] - Immunohistochemistry analysis using Mouse Anti-PSD-95 Monoclonal Antibody, Clone 7E3 (NBP2-12872). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-PSD-95 Monoclonal Antibody (NBP2-12872) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Basal cell staining in the epidermis, some hair follicle staining, dermal staining. Backskin obtained from transgenic mice. .

Immunocytochemistry/Immunofluorescence: PSD-95 Antibody (7E3) [NBP2-12872] - Mouse brain cultured neurons. Image from verified customer review.

Immunohistochemistry: PSD-95 Antibody (7E3) [NBP2-12872] - Tissue: Neocortex. Species: Rat. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody at 1:1000.

Immunohistochemistry-Frozen: PSD-95 Antibody (7E3) [NBP2-12872] - Mouse brain section (hippocampus). Image from verified customer review.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ICC/IF, IHC, MA
• Clone: 7E3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

PSD-95 Antibody (7E3) - BSA Free Summary

• Immunogen: Recombinant rat PSD-95
• Localization: Cell Membrane , Cell Junction , Synapse , Postsynaptic Cell Membrane , Postsynaptic Density , Cell Projection , Axon
• Marker: post-Synaptic Marker
• Specificity: Detects ~100 kDa. An additional protein of >100 kDa is also detected. Additional cross-reactive bands are detected at ~75 kDa and 50 kDa in rat and mouse samples.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: DLG4
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Microarray
  • Western Blot 1:1000
• Application Notes: 1 ug/mL was sufficient for detection of PSD-95 on 20 ug rat brain tissue extract by ECL immunoblot analysis using Goat Anti-Mouse IgG: HRP as the secondary. Use in IHC-Fr reported by customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for PSD-95 Antibody (7E3) - BSA Free

• discs, large homolog 4 (Drosophila)
• disks large homolog 4
• DLG4
• FLJ97752
• FLJ98574
• Postsynaptic density protein 95
• post-synaptic density protein 95
• PSD95
• PSD-95
• PSD95SAP90SAP-90discs large homolog 4
• SAP90
• Synapse-associated protein 90
• Tax interaction protein 15

Background

1ug/ml was sufficient for detection of PSD-95 on 20ug rat brain tissue extract by ECL immunoblot analysis using Goat Anti-Mouse igG: HRP as the secondary.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PSD-95 Antibody (NBP2-12872)(3)

Publications using NBP2-12872

• Munawar N, Bitar MS, Masocha W Activation of 5-HT1A Receptors Normalizes the Overexpression of Presynaptic 5-HT1A Receptors and Alleviates Diabetic Neuropathic Pain International journal of molecular sciences 2023-09-20 [PMID: 37762636] (WB, Rat)
• Guo X, Liu Y, Morgan D, Zhao LR. Reparative Effects of Stem Cell Factor and Granulocyte Colony-Stimulating Factor in Aged APP/PS1 Mice Aging and disease 2020-12-01 [PMID: 33269098]
• Qiu X, Ping S, Kyle M et al. Long-term beneficial effects of hematopoietic growth factors on brain repair in the chronic phase of severe traumatic brain injury Exp. Neurol. 2020-04-29 [PMID: 32360282] (ICC/IF, Mouse)

Review for PSD-95 Antibody (NBP2-12872) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Xuecheng Qiu IHC-Fr Mouse 05/12/2019

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Cell culture,Mouse brain
• Species: Mouse
• Lot: 1101

Bioinformatics

• Gene Symbol: DLG4
• Uniprot:
  • P31016(Rat)