PRPF4B Antibody

Western Blot: PRPF4B Antibody [NBP1-82999] - Analysis in human cell line HELA.

Immunocytochemistry/Immunofluorescence: PRPF4B Antibody [NBP1-82999] - Staining of human cell line A-431 shows localization to nuclear speckles. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: PRPF4B Antibody [NBP1-82999] - Staining of human urinary bladder shows strong nuclear and cytoplasmic positivity in urothelial cells.

Immunohistochemistry-Paraffin: PRPF4B Antibody [NBP1-82999] - Staining of human cerebellum shows moderate nuclear positivity in Purkinje cells.

Immunohistochemistry-Paraffin: PRPF4B Antibody [NBP1-82999] - Staining of human testis shows moderate nuclear positivity in Leydig cells.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

PRPF4B Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: LEDFDVEEEDEEALIEQRRIQRQAIVQKYKYLAEDSNMSVPSEPSSPQSSTRTRSPSPDDILERVAADVKEYERENVDTFEASVKAKHNLMTVEQNNGSSQKKLLAPDMF
• Predicted Species: Mouse (97%), Rat (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRPF4B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  PRPF4B Protein (NBP1-82999PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for PRPF4B Antibody

• dJ1013A10.1 (PRP4 protein kinase homolog)
• dJ1013A10.1
• KIAA0536EC 2.7.11.1
• PR4H
• protein-serine/threonine kinase
• PRP4 kinase
• PRP4 pre-mRNA processing factor 4 homolog B (yeast)
• PRP4 pre-mRNA-processing factor 4 homolog
• PRP4H
• PRP4K
• PRP4Prp4
• serine/threonine-protein kinase PRP4 homolog

Background

Pre-mRNA splicing occurs in two sequential transesterification steps, and the protein encoded by this gene is thought to be involved in pre-mRNA splicing and in signal transduction. This protein belongs to a kinase family that includes serine/arginine-rich protein-specific kinases and cyclin-dependent kinases (CDKs). This protein is regarded as a CDK-like kinase (Clk) with homology to mitogen-activated protein kinases (MAPKs). [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PRPF4B Antibody (NBP1-82999)(1)

Publication using NBP1-82999

• Clarke LE, Cook A, Mathavarajah S Et al. Haploinsufficient tumor suppressor PRP4K is negatively regulated during epithelial-to-mesenchymal transition FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2021-11-01 [PMID: 34674320] (WB)

Bioinformatics

• Gene Symbol: PRPF4B