PRPF4 Antibody - BSA Free

Western Blot: PRPF4 Antibody [NBP3-12635] - Lane A: Raji Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: 293 Whole Cell LysateLane D: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:58 kDaObserved band size:58 kDa

Immunoprecipitation: PRPF4 Antibody [NBP3-12635] - Lane A:0.5 mg 293 Whole Cell Lysate4 uL anti-PRPF4 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PRPF4 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 58 kDaObserved band size :58 kDa

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PRPF4 Antibody - BSA Free Summary

• Description: This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
  
  Protein A & Antigen Affinity purified
• Immunogen: Produced in rabbits immunized with E. coli-derived Human PRPF4 fragment
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRPF4
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1-5ul/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for PRPF4 Antibody - BSA Free

• hPrp4
• HPRP4P
• HPRP4PRP4/STK/WD splicing factor
• PRP4 homolog
• PRP4 pre-mRNA processing factor 4 homolog (yeast)
• Prp4p
• PRP4WD splicing factor Prp4
• U4/U6 small nuclear ribonucleoprotein Prp4
• U4/U6 snRNP 60 kDa protein

Background

The removal of introns from nuclear pre-mRNAs occurs on complexes called spliceosomes, which are made up of 4 small nuclear ribonucleoprotein (snRNP) particles and an undefined number of transiently associated splicing factors. PRPF4 is 1 of several proteins that associate with U4 and U6 snRNPs.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRPF4