PPIH Antibody

Images

 
Western Blot: PPIH Antibody [NBP2-19899] - Sample (30 ug of whole cell lysate) A: HeLa 12% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: PPIH Antibody [NBP2-19899] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: PPIH Antibody [NBP2-19899] - Mahlarvu xenograft PPIH antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Product Details

Summary
Reactivity Hu, RMSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

PPIH Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human PPIH. The exact sequence is proprietary.
Localization
Nucleus speckle; Cytoplasm
Predicted Species
Rhesus Macaque (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PPIH
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for PPIH Antibody

  • cyclophilin H
  • CYP20
  • CypH
  • CYPHpeptidyl prolyl isomerase H (cyclophilin H)
  • EC 5.2.1.8
  • MGC5016
  • peptidyl-prolyl cis-trans isomerase H
  • peptidylprolyl isomerase H (cyclophilin H)
  • PPIase H
  • Rotamase H
  • Small nuclear ribonucleoprotein particle-specific cyclophilin H
  • SnuCyp-20
  • USA-CyP SnuCyp-20
  • USA-CYPCYP-20
  • U-snRNP-associated cyclophilin SnuCyp-20
  • U-snRNP-associated cyclophilin SunCyp-20

Background

The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is a specific component of the complex that includes pre-mRNA processing factors PRPF3, PRPF4, and PRPF18, as well as U4/U5/U6 tri-snRNP. This protein has been shown to possess PPIase activity and may act as a protein chaperone that mediates the interactions between different proteins inside the spliceosome. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol PPIH
Uniprot