| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83218-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38223-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83217-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22606-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-12635-50ul | |||
![Western Blot: PRPF4 Antibody [NBP1-83218] - Analysis using Anti-PRPF4 antibody NBP1-83218 (A) shows similar pattern to independent antibody NBP1-83217 (B).](http://images.novusbio.com/fullsize/PRPF4-Antibody-Western-Blot-NBP1-83218-img0015.jpg "<b>Independent Antibodies Validation. </b>Western Blot: PRPF4 Antibody [NBP1-83218] - Analysis using Anti-PRPF4 antibody NBP1-83218 (A) shows similar pattern to independent antibody NBP1-83217 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: PRPF4 Antibody [NBP1-83218] - Staining of human cell line U-2 OS shows localization to nuclear speckles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PRPF4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83218-img0007.jpg)
![Immunohistochemistry: PRPF4 Antibody [NBP3-38223] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen using PRPF4 Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38223_rabbit-prpf4-pab-101220241224148.jpg "Immunohistochemistry: PRPF4 Antibody [NBP3-38223] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen using PRPF4 Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Immunohistochemistry: PRPF4 Antibody [NBP3-38223] - Immunohistochemistry analysis of paraffin-embedded Human brain using PRPF4 Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38223_rabbit-prpf4-pab-1012202411552881.jpg)
![Immunohistochemistry: PRPF4 Antibody [NBP3-38223] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer using PRPF4 Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38223_rabbit-prpf4-pab-10122024120682.jpg)
![Immunohistochemistry: PRPF4 Antibody [NBP3-38223] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using PRPF4 Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38223_rabbit-prpf4-pab-1012202411555915.jpg)
![Western Blot: PRPF4 Antibody [NBP3-38223] - Western blot analysis of various lysates using PRPF4 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-38223_rabbit-prpf4-pab-1012202411555918.jpg)
![Immunohistochemistry: PRPF4 Antibody [NBP3-38223] - Immunohistochemistry analysis of paraffin-embedded Rat lung using PRPF4 Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38223_rabbit-prpf4-pab-1012202412065.jpg)
![Western Blot: PRPF4 Antibody [NBP1-83217] - Analysis using Anti-PRPF4 antibody NBP1-83217 (A) shows similar pattern to independent antibody NBP1-83218 (B).](http://images.novusbio.com/fullsize/PRPF4-Antibody-Western-Blot-NBP1-83217-img0009.jpg "<b>Independent Antibodies Validation. </b>Western Blot: PRPF4 Antibody [NBP1-83217] - Analysis using Anti-PRPF4 antibody NBP1-83217 (A) shows similar pattern to independent antibody NBP1-83218 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: PRPF4 Antibody [NBP1-83217] - Staining of human cell line A-431 shows localization to nuclear speckles.](http://images.novusbio.com/fullsize/PRPF4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83217-img0007.jpg)
![Western Blot: PRPF4 Antibody [H00009128-B01P] - Analysis of PRPF4 expression in human spleen.](http://images.novusbio.com/fullsize/PRPF4-Antibody-Western-Blot-H00009128-B01P-img0005.jpg "Western Blot: PRPF4 Antibody [H00009128-B01P] - Analysis of PRPF4 expression in human spleen.")
![Immunocytochemistry/Immunofluorescence: PRPF4 Antibody [H00009128-B01P] - Analysis of purified antibody to PRPF4 on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/PRPF4-Antibody-Immunocytochemistry-Immunofluorescence-H00009128-B01P-img0004.jpg)
![Western Blot: PRPF4 Antibody [H00009128-B01P] - Analysis of PRPF4 expression in transfected 293T cell line by PRPF4 polyclonal antibody. Lane 1: PRPF4 transfected lysate(57.42 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PRPF4-Antibody-Western-Blot-H00009128-B01P-img0006.jpg)
![Western Blot: PRPF4 Antibody (JE65-37) [NBP3-32858] - Western blot analysis of PRPF4 on different lysates with Rabbit anti-PRPF4 antibody (NBP3-32858) at 1/500 dilution. Lane 1: HT-29 cell lysate Lane 2: SW480 cell lysate Lane 3: MCF-7 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 58 kDa Observed band size: 58 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32858) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32858_rabbit-prpf4-mab-je65-37-288202412442978.jpg "Western Blot: PRPF4 Antibody (JE65-37) [NBP3-32858] - Western blot analysis of PRPF4 on different lysates with Rabbit anti-PRPF4 antibody (NBP3-32858) at 1/500 dilution. Lane 1: HT-29 cell lysate Lane 2: SW480 cell lysate Lane 3: MCF-7 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 58 kDa Observed band size: 58 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32858) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PRPF4 Antibody (JE65-37) [NBP3-32858] - Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue with Rabbit anti-PRPF4 antibody (NBP3-32858) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32858) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32858_rabbit-prpf4-mab-je65-37-298202414333660.jpg)
![Immunocytochemistry/ Immunofluorescence: PRPF4 Antibody (JE65-37) [NBP3-32858] - Immunocytochemistry analysis of SiHa cells labeling PRPF4 with Rabbit anti-PRPF4 antibody (NBP3-32858) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PRPF4 antibody (NBP3-32858) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32858_rabbit-prpf4-mab-je65-37-292024119230.jpg)
![Western Blot: PRPF4 Antibody (SR2032) [NBP3-22606] - Western blot analysis using NBP3-22606 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-22606_rabbit-prpf4-mab-sr2032-57202316582194.jpg "Western Blot: PRPF4 Antibody (SR2032) [NBP3-22606] - Western blot analysis using NBP3-22606 in HeLa cell lysate.")
![Western Blot: PRPF4 Antibody [NBP3-12635] - Lane A: Raji Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: 293 Whole Cell LysateLane D: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:58 kDaObserved band size:58 kDa](http://images.novusbio.com/fullsize/PRPF4-Antibody-Western-Blot-NBP3-12635-img0001.jpg "Western Blot: PRPF4 Antibody [NBP3-12635] - Lane A: Raji Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: 293 Whole Cell LysateLane D: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:58 kDaObserved band size:58 kDa")
![Immunoprecipitation: PRPF4 Antibody [NBP3-12635] - Lane A:0.5 mg 293 Whole Cell Lysate4 uL anti-PRPF4 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PRPF4 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 58 kDaObserved band size :58 kDa](http://images.novusbio.com/fullsize/PRPF4-Antibody-Immunoprecipitation-NBP3-12635-img0002.jpg)
![Western Blot: PRPF4 Antibody [NBP2-19964] - Sample (30 ug of whole cell lysate) A: HeLa 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/PRPF4-Antibody-Western-Blot-NBP2-19964-img0001.jpg "Western Blot: PRPF4 Antibody [NBP2-19964] - Sample (30 ug of whole cell lysate) A: HeLa 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: PRPF4 Antibody [NBP1-57150] - NCI-H226 cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/PRPF4-Antibody-Western-Blot-NBP1-57150-img0003.jpg "Western Blot: PRPF4 Antibody [NBP1-57150] - NCI-H226 cell lysate, concentration 0.2-1 ug/ml.")
![Western Blot: PRPF4 Overexpression Lysate (Adult Normal) [NBL1-14821] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PRPF4.](http://images.novusbio.com/fullsize/PRPF4-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14821-img0002.jpg "Western Blot: PRPF4 Overexpression Lysate (Adult Normal) [NBL1-14821] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PRPF4.")