PRPF31 Antibodies
We offer PRPF31 Antibodies for use in common research applications: ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Sandwich ELISA, Western Blot. Each PRPF31 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our PRPF31 Antibodies can be used in a variety of model species: Human. Use the list below to choose the PRPF31 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our PRPF31 polyclonal antibodies and browse our PRPF31 monoclonal antibody catalog.
Alternate Names for PRPF31 Antibodies
anti-PRPF31 antibody, anti-DKFZp566J153 antibody, anti-hPrp31 antibody, anti-NY-BR-99 antibody, anti-Pre-mRNA-processing factor 31 antibody, anti-Protein 61K antibody, anti-PRP31 pre-mRNA processing factor 31 homolog (S. cerevisiae) antibody, anti-PRP31 pre-mRNA processing factor 31 homolog (yeast) antibody, anti-PRP31RP11 antibody, anti-Serologically defined breast cancer antigen NY-BR-99 antibody, anti-U4/U6 small nuclear ribonucleoprotein Prp31 antibody, anti-U4/U6 snRNP 61 kDa protein antibody
9 Results for "prpf31" in Primary Antibodies
![Western Blot: PRPF31 Antibody [NB100-55409] - Staining (1ug/ml) of A431 (A), HeLa (B) and HEK293 (C) nuclear cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PRPF31-Antibody-Western-Blot-NB100-55409-img0006.jpg "Western Blot: PRPF31 Antibody [NB100-55409] - Staining (1ug/ml) of A431 (A), HeLa (B) and HEK293 (C) nuclear cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: PRPF31 Antibody [NB100-55409] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong nuclear and some cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/PRPF31-Antibody-Immunocytochemistry-Immunofluorescence-NB100-55409-img0005.jpg)
![Flow Cytometry: PRPF31 Antibody [NB100-55409] - Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/PRPF31-Antibody-Flow-Cytometry-NB100-55409-img0003.jpg)
![Western Blot: PRPF31 Antibody [NB100-55409] - Staining (1ug/ml) of NIH3T3 nuclear cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PRPF31-Antibody-Western-Blot-NB100-55409-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: PRPF31 Antibody [NB100-55409] - Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong nuclear and weak cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/PRPF31-Antibody-Immunocytochemistry-Immunofluorescence-NB100-55409-img0004.jpg)
![Immunohistochemistry: PRPF31 Antibody [NBP3-38179] - Immunohistochemistry analysis of paraffin-embedded Human gastric cancer using PRPF31 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38179_rabbit-prpf31-pab-1012202411572625.jpg "Immunohistochemistry: PRPF31 Antibody [NBP3-38179] - Immunohistochemistry analysis of paraffin-embedded Human gastric cancer using PRPF31 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunohistochemistry: PRPF31 Antibody [NBP3-38179] - Immunohistochemistry analysis of paraffin-embedded Rat heart using PRPF31 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38179_rabbit-prpf31-pab-10122024121051.jpg)
![Western Blot: PRPF31 Antibody [NBP3-38179] - Western blot analysis of various lysates using PRPF31 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-38179_rabbit-prpf31-pab-101220241214489.jpg)
![Immunohistochemistry: PRPF31 Antibody [NBP3-38179] - Immunohistochemistry analysis of paraffin-embedded Mouse brain using PRPF31 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38179_rabbit-prpf31-pab-10122024120630.jpg)
![Western Blot: PRPF31 Antibody (JE64-82) [NBP3-32857] - Western blot analysis of PRPF31 on Jurkat cell lysates with Rabbit anti-PRPF31 antibody (NBP3-32857) at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 55 kDa Observed band size: 60 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32857) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32857_rabbit-prpf31-mab-je64-82-288202412442982.jpg "Western Blot: PRPF31 Antibody (JE64-82) [NBP3-32857] - Western blot analysis of PRPF31 on Jurkat cell lysates with Rabbit anti-PRPF31 antibody (NBP3-32857) at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 55 kDa Observed band size: 60 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32857) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PRPF31 Antibody (JE64-82) [NBP3-32857] - Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-PRPF31 antibody (NBP3-32857) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32857) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32857_rabbit-prpf31-mab-je64-82-298202414304132.jpg)
![Flow Cytometry: PRPF31 Antibody (JE64-82) [NBP3-32857] - Flow cytometric analysis of SiHa cells labeling PRPF31. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32857, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32857_rabbit-prpf31-mab-je64-82-29202416453218.jpg)
![Western Blot: PRPF31 Antibody (8E1) [H00026121-M02] - PRPF31 monoclonal antibody (M02), clone 8E1. Analysis of PRPF31 expression in human liver.](http://images.novusbio.com/fullsize/PRPF31-Antibody-8E1-Western-Blot-H00026121-M02-img0002.jpg "Western Blot: PRPF31 Antibody (8E1) [H00026121-M02] - PRPF31 monoclonal antibody (M02), clone 8E1. Analysis of PRPF31 expression in human liver.")
![Western Blot: PRPF31 Antibody (8E1) [H00026121-M02] - Analysis of PRPF31 expression in transfected 293T cell line by PRPF31 monoclonal antibody (M02), clone 8E1.Lane 1: PRPF31 transfected lysate(55.5 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PRPF31-Antibody-8E1-Western-Blot-H00026121-M02-img0003.jpg)
![Sandwich ELISA: PRPF31 Antibody (8E1) [H00026121-M02] - Detection limit for recombinant GST tagged PRPF31 is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PRPF31-Antibody-8E1-Sandwich-ELISA-H00026121-M02-img0001.jpg)
![Western Blot: PRPF31 Antibody (S01-7F1) [NBP3-19498] - Western blot detection of PRPF31 in C2C12, PC3 cell lysates using NBP3-19498 (1:1000 diluted). Predicted band size: 56kDa. Observed band size: 56kDa.](http://images.novusbio.com/fullsize/PRPF31-Antibody-S01-7F1-Western-Blot-NBP3-19498-img0002.jpg "Western Blot: PRPF31 Antibody (S01-7F1) [NBP3-19498] - Western blot detection of PRPF31 in C2C12, PC3 cell lysates using NBP3-19498 (1:1000 diluted). Predicted band size: 56kDa. Observed band size: 56kDa.")
![Immunocytochemistry/Immunofluorescence: PRPF31 Antibody (S01-7F1) [NBP3-19498] - Immunofluorescence of PRPF31 (green) in Hela using NBP3-19498 at dilution 1/50, and DAPI (blue)](http://images.novusbio.com/fullsize/PRPF31-Antibody-S01-7F1-Immunocytochemistry-Immunofluorescence-NBP3-19498-img0001.jpg)
![Western Blot: PRPF31 Antibody [NBP1-51909] - Analysis of PRPF31 in cell line A431 lysate using this antibody at 0.3 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PRPF31-Antibody-Western-Blot-NBP1-51909-img0002.jpg "Western Blot: PRPF31 Antibody [NBP1-51909] - Analysis of PRPF31 in cell line A431 lysate using this antibody at 0.3 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: PRPF31 Antibody [NBP1-51909] - (0.3ug/ml) staining of cell line A431 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PRPF31-Antibody-Western-Blot-NBP1-51909-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PRPF31 Antibody [NBP2-57695] - Staining of human cell line PC-3 shows localization to nucleus.](http://images.novusbio.com/fullsize/PRPF31-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57695-img0001.jpg "Immunocytochemistry/Immunofluorescence: PRPF31 Antibody [NBP2-57695] - Staining of human cell line PC-3 shows localization to nucleus.")
![Western Blot: PRPF31 Antibody [NBP1-82510] - Analysis in human cell line HEK 293.](http://images.novusbio.com/fullsize/PRPF31-Antibody-Western-Blot-NBP1-82510-img0001.jpg "Western Blot: PRPF31 Antibody [NBP1-82510] - Analysis in human cell line HEK 293.")
![Western Blot: PRPF31 Antibody [NBP2-19963] - Sample (30 ug of whole cell lysate) A: NT2D1 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/PRPF31-Antibody-Western-Blot-NBP2-19963-img0001.jpg "Western Blot: PRPF31 Antibody [NBP2-19963] - Sample (30 ug of whole cell lysate) A: NT2D1 7. 5% SDS PAGE gel, diluted at 1:1000.")