Prox1 Antibody [Unconjugated]

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Prox1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2727) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Prox1 at approximately 83 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Prox1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human Prox1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2727) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (left panel, red; Catalog # NL001) and counterstained with DAPI (right panel, blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Prox1 at approximately 114 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Prox1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2727) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

RNA-seq identifies the targets of GATA2 in primary human LECs. (A) Principal component analysis (PCA) was performed on RNA-seq data from control shRNA- and shGATA2-infected primary HLECs. A high level of similarity was observed within the groups as indicated by their proximity to each other. (B) Hierarchical clustering shows that approximately 1000 genes were consistently downregulated and 600 genes were upregulated in shGATA2-treated HLECs. (C) GO revealed a list of genes that are likely relevant to the phenotypes observed in mice lacking GATA2. (D) GATA2 was knocked out from a second HLEC line using CRISPR/Cas9. Western blot revealed the lack of GATA2 in the knockout cells (HLEC delta GATA2). In contrast, no obvious differences were observed in the expression of PROX1. Additionally, qRT-PCR revealed the downregulation of miR-126. (A) n=3 independent experiments per shRNA; (D) n=3 independent experiments (antibiotic selection, western blot and qRT-PCR). **P<0.01. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31582413), licensed under a CC-BY license. Not internally tested by R&D Systems.

Prox1 regulates myoblast differentiation.(a) Analysis of Prox1 mRNA and protein during C2C12 myoblast differentiation (indicated by the increase in MyoD1 mRNA). (b) Prox1 protein and mRNA in human myoblasts before and after differentiation. Primary myoblast lines from two different individuals were analysed. (c–f) Analysis of myoblast proliferation and differentiation-related CyclinD1, Myf5 and MyoD mRNAs in shProx1 and shScr transduced primary human myoblasts before (c) and after differentiation for 7d (d), as well as in mouse C2C12 myoblasts before (e) and after (f) differentiation. (g) Myosin staining of C2C12 cells after 7d of differentiation. Note that Prox1 silencing completely blocked myotube development, as only occasional cells expressed myosin and these cells were negative for shProx1-GFP. Data is presented as mean±s.e.m., n=3+3 biological replicates in a–g (all experiments were repeated three times). One-way analysis of variance with repeated measures followed by Tukey's posthoc test and Student's two-tailed unpaired t-test, *P<0.05, **P<0.01, ***P<0.001. Scale bars, 50 μm. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms13124), licensed under a CC-BY license. Not internally tested by R&D Systems.

In situ localization of Ptx3-LECs and transition between Ptx3-LECs and Marco-LECs in human LNs. (A) Expression of CD36/Cd36 in LN LEC subsets of human and mouse. Dots indicate mean log-normalized transcript count. (B–D) Identification of CD36high Ptx3-LECs in human head and neck LNs by immunostaining. (B,C) Immunofluorescence of PROX-1, MARCO and CD36 (B), or PROX-1, LYVE-1 and CLEC4M (C). Zoomed-in images (inset marked by blue dotted lines) in (B) and (C) demonstrate CD36high LYVE-1+ paracortical sinuses (filled arrowhead). Scale bars = 500 μm (left panels) and 100 μm (right panel inset). (D) CD36high LYVE-1+ Ptx3-LECs (filled arrowhead) can be seen associated with MARCO+ CLEC4M+ Marco-LECs (empty arrowhead) in human LNs. Scale bars = 100 μm. CD36high Ptx3-LECs were detected in four out of seven human LNs. Images are representative of four biological replicates. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32426372), licensed under a CC-BY license. Not internally tested by R&D Systems.

In situ localization of Ptx3-LECs and transition between Ptx3-LECs and Marco-LECs in human LNs. (A) Expression of CD36/Cd36 in LN LEC subsets of human and mouse. Dots indicate mean log-normalized transcript count. (B–D) Identification of CD36high Ptx3-LECs in human head and neck LNs by immunostaining. (B,C) Immunofluorescence of PROX-1, MARCO and CD36 (B), or PROX-1, LYVE-1 and CLEC4M (C). Zoomed-in images (inset marked by blue dotted lines) in (B) and (C) demonstrate CD36high LYVE-1+ paracortical sinuses (filled arrowhead). Scale bars = 500 μm (left panels) and 100 μm (right panel inset). (D) CD36high LYVE-1+ Ptx3-LECs (filled arrowhead) can be seen associated with MARCO+ CLEC4M+ Marco-LECs (empty arrowhead) in human LNs. Scale bars = 100 μm. CD36high Ptx3-LECs were detected in four out of seven human LNs. Images are representative of four biological replicates. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32426372), licensed under a CC-BY license. Not internally tested by R&D Systems.

Prox1 regulates the slow muscle fibre gene program in skeletal muscle.(a) MyHC I (Myh7) expression in muscle fibres (arrows) in AAV8-Prox1 and AAV8-Ctrl transduced TA muscle. (b) Expression of myosin heavy chain and calcium-signalling genes in AAV8-Prox1 and AAV8-Ctrl transduced TA muscle. Note that the expression of slow fibre -specific genes are upregulated and fast MyHC genes downregulated in Prox1 overexpressing muscles. (c) Heat map from GSEA analysis showing that Prox1 overexpression in TA muscle leads to expression of many soleus specific myofibrillar genes (red colour indicates positive and blue negative enrichment). (d) Effect of Prox1 deletion on soleus muscle gene expression (HSA-CreERT2;Prox1fl/fl mice). Note the increased expression of fast MyHCs Myh2 and Myh4 RNAs, whereas slow troponin isoform RNAs and Myh7 RNA are decreased. (e) Deletion of Prox1 in slow soleus muscle with AAV-Cre leads to marked downregulation of MyHC I protein. Data is presented as mean±s.e.m., n=5+5 in a,b, n=3+3 in c, n=4+4 in d,e. Student's two-tailed unpaired t-test, *P<0.05, **P<0.01, ***P<0.001. Both overexpression and deletion experiments were conducted two times on independent mouse cohorts. WT, wild-type. Scale bars, 50 μm. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms13124), licensed under a CC-BY license. Not internally tested by R&D Systems.

In situ localization of Ptx3-LECs and transition between Ptx3-LECs and Marco-LECs in human LNs. (A) Expression of CD36/Cd36 in LN LEC subsets of human and mouse. Dots indicate mean log-normalized transcript count. (B–D) Identification of CD36high Ptx3-LECs in human head and neck LNs by immunostaining. (B,C) Immunofluorescence of PROX-1, MARCO and CD36 (B), or PROX-1, LYVE-1 and CLEC4M (C). Zoomed-in images (inset marked by blue dotted lines) in (B) and (C) demonstrate CD36high LYVE-1+ paracortical sinuses (filled arrowhead). Scale bars = 500 μm (left panels) and 100 μm (right panel inset). (D) CD36high LYVE-1+ Ptx3-LECs (filled arrowhead) can be seen associated with MARCO+ CLEC4M+ Marco-LECs (empty arrowhead) in human LNs. Scale bars = 100 μm. CD36high Ptx3-LECs were detected in four out of seven human LNs. Images are representative of four biological replicates. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32426372), licensed under a CC-BY license. Not internally tested by R&D Systems.

TLS formation in models of heart failure, conditional lymphatic-EC deletion of Rbpj or kidney ischemia reperfusion. D PAS staining of representative, paraffin-embedded kidney sections. Quantification of infiltrated area [in mm2] per transversal kidney cross-section (sum of all infiltrated areas per section), N = 8 biological replicates per group, Mann–Whitney test, two-tailed, p = 0.51. Graph: Scatter dot blot, Mean, SD (whiskers). ERbpj delta EC Whole kidney staining for B220 (TLS)&Prox1/Lyve1 for lymphatic collecting vessels, light sheet microscopy, ventral view, 3D reconstruction via IMARIS software; scale bar: left image 1000 µm; insets are magnifications of boxed detail, scale bar 150 µm. Exemplary image, kidneys from N = 3 mice stained. F Induction protocol for lymphatic endothelial-restricted deletion of Rbpj (Rbpj delta LEC). Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35440634), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, Simple Western, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Prox1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Prox1
  Pro2-Gln259
  Accession # Q92786
• Specificity: Detects human Prox1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PROX1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Prox1 Antibody [Unconjugated]

• Homeobox prospero-like protein PROX1
• prospero homeobox 1
• prospero homeobox protein 1
• prospero-related homeobox 1
• Prox1
• PROX-1

Background

Prox1, the homologue of Drosophila Prospero, is a homeobox-containing transcription factor that binds and functions as a co-receptor of liver receptor homolog 1 (LRH1/NR5A2). It is a specific marker for lymphatic endothelial cells as well as hepatoblasts and hepatocytes. Human Prox1 shares 97% amino acid sequence homology with mouse and rat Prox1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Prox1 Antibody (AF2727)(152)

Publications using AF2727

• Fernandes, LM;Griswold-Wheeler, D;Tresemer, JD;Vallejo, A;Vishlaghi, N;Levi, B;Shapiro, A;Scallan, JP;Dellinger, MT; A single-cell atlas of normal and KRASG12D-malformed lymphatic vessels JCI insight 2025-01-28 [PMID: 39874106] (Immunohistochemistry, Mouse)
• Spurgin, S;Nguimtsop, AM;Chaudhry, FN;Michki, SN;Salvador, J;Iruela-Arispe, ML;Zepp, JA;Mukhopadhyay, S;Cleaver, O; Spatiotemporal dynamics of primary and motile cilia throughout lung development bioRxiv : the preprint server for biology 2024-10-26 [PMID: 39484464] (Immunohistochemistry, Transgenic Mouse)
• Nouruzi, S;Namekawa, T;Tabrizian, N;Kobelev, M;Sivak, O;Scurll, JM;Cui, CJ;Ganguli, D;Zoubeidi, A; ASCL1 regulates and cooperates with FOXA2 to drive terminal neuroendocrine phenotype in prostate cancer JCI insight 2024-12-06 [PMID: 39470735] (Western Blot, Human)
• Crossey, E;Carty, S;Shao, F;Henao-Vasquez, J;Ysasi, AB;Zeng, M;Hinds, A;Lo, M;Tilston-Lunel, A;Varelas, X;Jones, MR;Fine, A; Influenza induces lung lymphangiogenesis independent of YAP/TAZ activity in lymphatic endothelial cells. Scientific reports 2024-09-12 [PMID: 39266641] (Immunohistochemistry, Mouse)
• Arroyo-Ataz, G;Yagüe, AC;Breda, JC;Mazzilli, SA;Jones, D; Transcriptional, developmental, and functional parallels of lymphatic and venous smooth muscle bioRxiv : the preprint server for biology 2024-07-22 [PMID: 39091770] (Immunohistochemistry, Transgenic Mouse)
• Manolis, D;Hasan, S;Maraveyas, A;O'Brien, DP;Kessler, BM;Kramer, H;Nikitenko, LL; Quantitative proteomics reveals CLR interactome in primary human cells The Journal of biological chemistry 2024-05-20 [PMID: 38777147] (Immunocytochemistry, Human)
• Crossey, E;Carty, S;Shao, F;Henao-Vasquez, J;Ysasi, AB;Zeng, M;Hinds, A;Lo, M;Tilston-Lunel, A;Varelas, X;Jones, MR;Fine, A; Influenza Induces Lung Lymphangiogenesis Independent of YAP/TAZ Activity in Lymphatic Endothelial Cells Research square 2024-02-27 [PMID: 38463972] (Immunohistochemistry, Mouse)
• Sun, Q;Li, J;Ma, J;Zheng, Y;Ju, R;Li, X;Ren, X;Huang, L;Chen, R;Tan, X;Luo, L; JAM-C Is Important for Lens Epithelial Cell Proliferation and Lens Fiber Maturation in Murine Lens Development Investigative ophthalmology & visual science 2023-12-01 [PMID: 38095908] (IHC, Mouse)
• Agnes Lee Chen Ong, Toshiya Kokaji, Arisa Kishi, Yoshihiro Takihara, Takuma Shinozuka, Ren Shimamoto et al. Acquisition of neural fate by combination of BMP blockade and chromatin modification iScience 2023-10-20 [PMID: 37771660]
• Swati Jain, John J. LaFrancois, Kasey Gerencer, Justin J. Botterill, Meghan Kennedy, Chiara Criscuolo et al. Increasing adult neurogenesis protects mice from epilepsy bioRxiv 2023-07-14 [PMID: 37502909]
• Jannaway, M;Iyer, D;Mastrogiacomo, DM;Li, K;Sung, DC;Yang, Y;Kahn, ML;Scallan, JP; VEGFR3 is required for button junction formation in lymphatic vessels Cell reports 2023-07-15 [PMID: 37454290] (IHC, Mouse)
• Ohata, H;Shiokawa, D;Sakai, H;Kanda, Y;Okimoto, Y;Kaneko, S;Hamamoto, R;Nakagama, H;Okamoto, K; PROX1 induction by autolysosomal activity stabilizes persister-like state of colon cancer via feedback repression of the NOX1-mTORC1 pathway Cell reports 2023-05-17 [PMID: 37224811] (IHC, Xenograft, Human)
• PV Blinkiewic, MR Long, ZA Stoner, EM Ketchum, SN Sheltz-Kem, JS Duncan Gata3 is Required in Late Proneurosensory Development for Proper Sensory Cell Formation and Organization Research square, 2023-04-14;0(0):. 2023-04-14 [PMID: 37090645] (IHC, Mouse)
• D Greene, Genomics E, D Pirri, K Frudd, E Sackey, M Al-Owain, APJ Giese, K Ramzan, S Riaz, I Yamanaka, N Boeckx, C Thys, BD Gelb, P Brennan, V Hartill, J Harvengt, T Kosho, S Mansour, M Masuno, T Ohata, H Stewart, K Taibah, CLS Turner, F Imtiaz, S Riazuddin, T Morisaki, P Ostergaard, BL Loeys, H Morisaki, ZM Ahmed, GM Birdsey, K Freson, A Mumford, E Turro Genetic association analysis of 77,539 genomes reveals rare disease etiologies Nature Medicine, 2023-03-16;29(3):679-688. 3/16/2023 [PMID: 36928819]
• Ivy K N Chiang, Matthew S Graus, Nils Kirschnick, Tara Davidson, Winnie Luu, Richard Harwood et al. The blood vasculature instructs lymphatic patterning in a SOX7‐dependent manner The EMBO Journal 2023-03-01 [PMID: 36715213] (Immunohistochemistry, Mouse)
• J Kazenwadel, P Venugopal, A Oszmiana, J Toubia, L Arriola-Ma, V Panara, SG Piltz, C Brown, W Ma, AW Schreiber, K Koltowska, S Taoudi, PQ Thomas, HS Scott, NL Harvey A Prox1 enhancer represses haematopoiesis in the lymphatic vasculature Nature, 2023-01-25;614(7947):343-348. 2023-01-25 [PMID: 36697821] (IHC, Mouse)
• Guillermo Arroyo-Ataz, Dennis Jones Dissection of murine collecting lymphatic vessels for imaging, single-cell analysis, and tissue culture of lymphatic muscle cells STAR Protocols 2022-12-01 [PMID: 36353362] (Immunohistochemistry, Mouse)
• Fernando Bonet, Sabrina Brito Añez, José Manuel Inácio, Matthias E. Futschik, José Antonio Belo CCBE1 Is Essential for Epicardial Function during Myocardium Development International Journal of Molecular Sciences 2022-10-20 [PMID: 36293499]
• Wakao S, Oguma Y, Kushida Y et al. Phagocytosing differentiated cell-fragments is a novel mechanism for controlling somatic stem cell differentiation within a short time frame Research Square 3/8/2022 [PMID: 36203068]
• Kazuaki Maruyama, Sachiko Miyagawa-Tomita, Yuka Haneda, Mayuko Kida, Fumio Matsuzaki, Kyoko Imanaka-Yoshida et al. The cardiopharyngeal mesoderm contributes to lymphatic vessel development in mouse eLife 2022-10-05 [PMID: 36196867]
• SH Lee, N Kim, M Kim, SH Woo, I Han, J Park, K Kim, KS Park, K Kim, D Shim, SE Park, JY Zhang, DM Go, DY Kim, WK Yoon, SP Lee, J Chung, KW Kim, JH Park, SH Lee, S Lee, SJ Ann, SH Lee, HS Ahn, SC Jeong, TK Kim, GT Oh, WY Park, HO Lee, JH Choi Single-cell transcriptomics reveal cellular diversity of aortic valve and the immunomodulation by PPARgamma during hyperlipidemia Nature Communications, 2022-09-17;13(1):5461. 2022-09-17 [PMID: 36115863] (IHC, Mouse)
• E Place, E Manning, DW Kim, A Kinjo, G Nakamura, K Ohyama SHH and Notch regulate SOX9+ progenitors to govern arcuate POMC neurogenesis Frontiers in Neuroscience, 2022-08-11;16(0):855288. 2022-08-11 [PMID: 36033614] (ICC/IF, Chicken)
• Y Zhou, Y Su, S Li, BC Kennedy, DY Zhang, AM Bond, Y Sun, F Jacob, L Lu, P Hu, AN Viaene, I Helbig, SK Kessler, T Lucas, RD Salinas, X Gu, HI Chen, H Wu, JE Kleinman, TM Hyde, DW Nauen, DR Weinberger, GL Ming, H Song Molecular landscapes of human hippocampal immature neurons across lifespan Nature, 2022-07-06;0(0):. 7/6/2022 [PMID: 35794479]
• FK Wong, M Selten, C Rosés-Nove, V Sreenivasa, N Pallas-Baz, E Serafeimid, A Hanusz-God, F Oozeer, R Edwards, O Marín Serotonergic regulation of bipolar cell survival in the developing cerebral cortex Cell Reports, 2022-07-05;40(1):111037. 2022-07-05 [PMID: 35793629] (IHC, Mouse)
• Laurent Jacob, Jose de Brito Neto, Stephanie Lenck, Celine Corcy, Farhat Benbelkacem, Luiz Henrique Geraldo et al. Conserved meningeal lymphatic drainage circuits in mice and humans Journal of Experimental Medicine 2022-08-01 [PMID: 35776089]
• AE Hall, SÖ Pohl, P Cammareri, S Aitken, NT Younger, M Raponi, CV Billard, AB Carrancio, A Bastem, P Freile, F Haward, IR Adams, JF Caceres, P Preyzner, A von Kriegs, MG Dunlop, FV Din, KB Myant RNA splicing is a key mediator of tumour cell plasticity and a therapeutic vulnerability in colorectal cancer Nature Communications, 2022-05-19;13(1):2791. 2022-05-19 [PMID: 35589755] (IHC, Western Blot, Mouse)
• A Choudhury, ST Magill, CD Eaton, BC Prager, WC Chen, MA Cady, K Seo, CG Lucas, TJ Casey-Clyd, HN Vasudevan, SJ Liu, JE Villanueva, TC Lam, JK Pu, LF Li, GK Leung, DL Swaney, MY Zhang, JW Chan, Z Qiu, MV Martin, MS Susko, SE Braunstein, NAO Bush, JD Schulte, N Butowski, PK Sneed, MS Berger, NJ Krogan, A Perry, JJ Phillips, DA Solomon, JF Costello, MW McDermott, JN Rich, DR Raleigh Meningioma DNA methylation groups identify biological drivers and therapeutic vulnerabilities Nature Genetics, 2022-05-09;54(5):649-659. 2022-05-09 [PMID: 35534562] (IHC, Human, Xenograft)
• I Genescu, M Aníbal-Mar, V Kouskoff, N Chenouard, C Mailhes-Ha, H Cartonnet, L Lokmane, FM Rijli, G López-Bend, F Gambino, S Garel Dynamic interplay between thalamic activity and Cajal-Retzius cells regulates the wiring of cortical layer 1 Cell Reports, 2022-04-12;39(2):110667. 2022-04-12 [PMID: 35417707] (IHC, Mouse)
• Xu J, Xu L, Sui P et al. Excess neuropeptides in lung signal through endothelial cells to impair gas exchange Developmental cell 3/11/2022 [PMID: 35303432]
• Maxence Cornille, Stéphanie Moriceau, Roman H. Khonsari, Yann Heuzé, Léa Loisay, Valérie Boitez et al. FGFR3 overactivation in the brain is responsible for memory impairments in Crouzon syndrome mouse model J Exp Med 2022-03-07 [PMID: 35254402] (Immunohistochemistry, Mouse)
• MC Friess, I Kritikos, P Schineis, JD Medina-San, AO Gkountidi, A Vallone, EC Sigmund, C Schwitter, M Vranova, C Matti, J Arasa, C Saygili De, E Bovay, ST Proulx, M Tomura, A Rot, DF Legler, TV Petrova, C Halin Mechanosensitive ACKR4 scavenges CCR7 chemokines to facilitate T�cell de-adhesion and passive transport by flow in inflamed afferent lymphatics Cell Reports, 2022-02-01;38(5):110334. 2022-02-01 [PMID: 35108538] (IHC, Mouse)
• Peter Baluk, Donald M. McDonald Imaging Blood Vessels and Lymphatics in Mouse Trachea Wholemounts Methods in Molecular Biology 2022-09-06 [PMID: 35099733]
• Takahiro Wakizono, Hideyuki Nakashima, Tetsuro Yasui, Teppei Noda, Kei Aoyagi, Kanako Okada et al. Growth factors with valproic acid restore injury-impaired hearing by promoting neuronal regeneration JCI Insight 2021-11-22 [PMID: 34806649]
• Rafael S. Czepielewski, Emma C. Erlich, Emily J. Onufer, Shannon Young, Brian T. Saunders, Yong-Hyun Han et al. Ileitis-associated tertiary lymphoid organs originate at lymphatic valves and obstruct mesenteric lymph flow in response to tumor necrosis factor Immunity 2021-12-01 [PMID: 34788601]
• Wang T, Wang Z, de Fabritus L Et al. 1-deoxysphingolipids bind to COUP-TF to modulate lymphatic and cardiac cell development Developmental cell 11/22/2021 [PMID: 34762852]
• D Olmeda, D Cerezo-Wal, C Mucientes, TG Calvo, E Cañón, D Alonso-Cur, N Ibarz, J Muñoz, JL Rodriguez-, P Ortiz-Rome, S Ortega, MS Soengas Live imaging of neolymphangiogenesis identifies acute antimetastatic roles of dsRNA mimics Embo Molecular Medicine, 2021-11-11;0(0):e12924. 2021-11-11 [PMID: 34762341] (IHC, Mouse)
• BR Thomson, P Liu, T Onay, J Du, SW Tompson, S Misener, RR Purohit, TL Young, J Jin, SE Quaggin Cellular crosstalk regulates the aqueous humor outflow pathway and provides new targets for glaucoma therapies Nature Communications, 2021-10-18;12(1):6072. 2021-10-18 [PMID: 34663817] (IHC, Mouse)
• NN Nawarawong, KR Thompson, SP Guerin, C Anasooya S, H Peng, K Nixon Reactive, Adult Neurogenesis From Increased Neural Progenitor Cell Proliferation Following Alcohol Dependence in Female Rats Frontiers in Neuroscience, 2021-09-14;15(0):689601. 2021-09-14 [PMID: 34594180] (IHC, Rat)
• E Redder, N Kirschnick, S Bobe, R Hägerling, NR Hansmeier, F Kiefer Vegfr3-tdTomato, a reporter mouse for microscopic visualization of lymphatic vessel by multiple modalities PLoS ONE, 2021-09-20;16(9):e0249256. 2021-09-20 [PMID: 34543279] (IHC, Mouse)
• Masahiro Otsu, Zubair Ahmed, Daniel Fulton Generation of Multipotential NG2 Progenitors From Mouse Embryonic Stem Cell-Derived Neural Stem Cells Frontiers in Cell and Developmental Biology 2021-08-24 [PMID: 34504841] (Immunocytochemistry, Mouse)
• Tevye Jason Stachniak, Rahel Kastli, Olivia Hanley, Ali Özgür Argunsah, Elianne Grietje Theodora van der Valk, George Kanatouris et al. Postmitotic Prox1 Expression Controls the Final Specification of Cortical VIP Interneuron Subtypes The Journal of Neuroscience 2021-09-29 [PMID: 34380763]
• M Uemura, S Blankvoort, SSL Tok, L Yuan, LF Cobar, KK Lit, A Tashiro A neurogenic microenvironment defined by excitatory-inhibitory neuronal circuits in adult dentate gyrus Cell Reports, 2021-07-06;36(1):109324. 2021-07-06 [PMID: 34233196] (IHC, Mouse)
• Sara E. Billings, Nina M. Myers, Lee Quiruz, Alan G. Cheng Opposing effects of Wnt/ beta -catenin signaling on epithelial and mesenchymal cell fate in the developing cochlea Development 2021-06-01 [PMID: 34061174]
• Delayna Paulson, Rebecca Harms, Cody Ward, Mackenzie Latterell, Gregory J. Pazour, Darci M. Fink Loss of Primary Cilia Protein IFT20 Dysregulates Lymphatic Vessel Patterning in Development and Inflammation Frontiers in Cell and Developmental Biology 2021-05-14 [PMID: 34055805]
• Wanshu Ma, Hyea Jin Gil, Xiaolei Liu, Lauren P. Diebold, Marc A. Morgan, Michael J. Oxendine-Burns et al. Mitochondrial respiration controls the Prox1-Vegfr3 feedback loop during lymphatic endothelial cell fate specification and maintenance Science Advances 2021-04-30 [PMID: 33931446]
• J Schaller, HM Hajjami, S Rusakiewic, K Ioannidou, N Piazzon, A Miles, D Golshayan, O Gaide, D Hohl, DE Speiser, K Schaeuble Mutually exclusive lymphangiogenesis or perineural infiltration in human skin squamous-cell carcinoma Oncotarget, 2021-03-30;12(7):638-648. 2021-03-30 [PMID: 33868585] (IHC, Human)
• G Limoni, S Murthy, D Jabaudon, A Dayer, M Niquille PlexinA4-Semaphorin3A-mediated crosstalk between main cortical interneuron classes is required for superficial interneuron lamination Cell Reports, 2021-01-26;34(4):108644. 2021-01-26 [PMID: 33503438] (IHC, Mouse, Transgenic Mouse)
• I Tai-Nagara, Y Hasumi, D Kusumoto, H Hasumi, K Okabe, T Ando, F Matsuzaki, F Itoh, H Saya, C Liu, W Li, YS Mukouyama, W Marston Li, X Liu, M Hirashima, Y Suzuki, S Funasaki, Y Satou, M Furuya, M Baba, Y Kubota Blood and lymphatic systems are segregated by the FLCN tumor suppressor Nature Communications, 2020-12-09;11(1):6314. 2020-12-09 [PMID: 33298956] (IHC, Mouse)
• Motawe Z Y, Abdelmaboud S S et al. Involvement of Sigma Receptor-1 in Lymphatic Endothelial Barrier Integrity and Bioenergetic Regulation. Lymphat Res Biol 2024-06-15 [PMID: 33226886] (Simple Western, Human)
  
  Details:
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• A Rattner, CE Terrillion, C Jou, T Kleven, SF Hu, J Williams, Z Hou, M Aggarwal, S Mori, G Shin, LA Goff, MP Witter, M Pletnikov, AA Fenton, J Nathans Developmental, cellular, and behavioral phenotypes in a mouse model of congenital hypoplasia of the dentate gyrus Elife, 2020-10-21;9(0):. 2020-10-21 [PMID: 33084572] (IHC, Mouse)
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• Park D, Lee J, Park I, Choi D, Lee S, Song S, Hwang Y, Hong K, Nakaoka Y, Makinen T, Kim P, Alitalo K, Hong Y, Koh G Lymphatic regulator PROX1 determines Schlemm&#039;s canal integrity and identity. J Clin Invest, 2014-07-25;124(9):3960-74. 2014-07-25 [PMID: 25061877] (IHC, Mouse)
• Zheng W, Nurmi H, Appak S, Sabine A, Bovay E, Korhonen E, Orsenigo F, Lohela M, D'Amico G, Holopainen T, Leow C, Dejana E, Petrova T, Augustin H, Alitalo K Angiopoietin 2 regulates the transformation and integrity of lymphatic endothelial cell junctions. Genes Dev, 2014-07-15;28(14):1592-603. 2014-07-15 [PMID: 25030698] (IHC-Fr, Mouse)
• Fatima A, Culver A, Culver F, Liu T, Dietz W, Thomson B, Hadjantonakis A, Quaggin S, Kume T Murine Notch1 is required for lymphatic vascular morphogenesis during development. Dev Dyn, 2014-04-17;243(7):957-64. 2014-04-17 [PMID: 24659232] (IHC, Mouse)
• Brzezinski J, Uoon Park K, Reh T Blimp1 (Prdm1) prevents re-specification of photoreceptors into retinal bipolar cells by restricting competence. Dev Biol, 2013-10-12;384(2):194-204. 2013-10-12 [PMID: 24125957] (IHC-Fr, Mouse)
• Sugiyama T, Osumi N, Katsuyama Y The germinal matrices in the developing dentate gyrus are composed of neuronal progenitors at distinct differentiation stages. Dev Dyn, 2013-10-07;242(12):1442-53. 2013-10-07 [PMID: 24038449] (IHC, Mouse)
• Francius C, Harris A, Rucchin V et al. Identification of Multiple Subsets of Ventral Interneurons and Differential Distribution along the Rostrocaudal Axis of the Developing Spinal Cord. PLoS One. 8/15/2013 [PMID: 23967072]
• Justin A. McClain, Stephanie A. Morris, S. Alexander Marshall, Kimberly Nixon Ectopic hippocampal neurogenesis in adolescent male rats following alcohol dependence Addiction Biology 2014-07-01 [PMID: 23844726]
• Sandrine Levet, Delphine Ciais, Galina Merdzhanova, Christine Mallet, Teresa A. Zimmers, Se-Jin Lee et al. Bone morphogenetic protein 9 (BMP9) controls lymphatic vessel maturation and valve formation Blood 2013-07-25 [PMID: 23741013]
• Hagerling R, Pollmann C, Andreas M, Schmidt C, Nurmi H, Adams R, Alitalo K, Andresen V, Schulte-Merker S, Kiefer F A novel multistep mechanism for initial lymphangiogenesis in mouse embryos based on ultramicroscopy. EMBO J, 2013-01-08;32(5):629-44. 2013-01-08 [PMID: 23299940] (IHC, Mouse)
• Aberrant mural cell recruitment to lymphatic vessels and impaired lymphatic drainage in a murine model of pulmonary fibrosis. Blood, 2012-04-30;119(24):5931-42. 2012-04-30 [PMID: 22547584] (ICC, IHC-P, Mouse)
• Mcl-1 and Bcl-x(L) coordinately regulate megakaryocyte survival. Blood, 2012-02-28;119(24):5850-8. 2012-02-28 [PMID: 22374700] (IHC, Mouse)
• Lara Planas-Paz, Boris Strilić, Axel Goedecke, Georg Breier, Reinhard Fässler, Eckhard Lammert Mechanoinduction of lymph vessel expansion The EMBO Journal 2012-02-15 [PMID: 22157817] (Immunohistochemistry, Mouse)
• Skog M, Bono P, Lundin M, Lundin J, Louhimo J, Linder N, Petrova TV, Andersson LC, Joensuu H, Alitalo K, Haglund CH Expression and prognostic value of transcription factor PROX1 in colorectal cancer. Br. J. Cancer, 2011-10-04;105(9):1346-51. 2011-10-04 [PMID: 21970873] (IHC-P, Human)
• Elyada E, Pribluda A, Goldstein RE, Morgenstern Y, Brachya G, Cojocaru G, Snir-Alkalay I, Burstain I, Haffner-Krausz R, Jung S, Wiener Z, Alitalo K, Oren M, Pikarsky E, Ben-Neriah Y CKIalpha ablation highlights a critical role for p53 in invasiveness control. Nature, 2011-02-17;470(7334):409-13. 2011-02-17 [PMID: 21331045] (IHC-P, Western Blot, Mouse)
• Xiaoren Chen, Tapan P. Patel, Vladimir I. Simirskii, Melinda K. Duncan PCNA interacts with Prox1 and represses its transcriptional activity Mol Vis 2008-11-17 [PMID: 19023449]
• Philipp M, Fralish GB, Meloni AR, Chen W, MacInnes AW, Barak LS, Caron MG Smoothened signaling in vertebrates is facilitated by a G protein-coupled receptor kinase. Mol. Biol. Cell, 2008-09-24;19(12):5478-89. 2008-09-24 [PMID: 18815277] (IHC, Zebrafish)
• Heckman CA, Holopainen T, Wirzenius M, Keskitalo S, Jeltsch M, Yla-Herttuala S, Wedge SR, Jurgensmeier JM, Alitalo K The tyrosine kinase inhibitor cediranib blocks ligand-induced vascular endothelial growth factor receptor-3 activity and lymphangiogenesis. Cancer Res., 2008-06-15;68(12):4754-62. 2008-06-15 [PMID: 18559522] (ICC, Human)

Reviews for Prox1 Antibody (AF2727) (4)

Average Rating: 4.8

(Based on 4 reviews)

We have 4 reviews tested in 4 species: Human, Mouse, Other, Rabbit.

reviewed by: Verified Customer IHC-Fr Rabbit 07/07/2020

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: brain hippocampus section,Rabbit Brain dentate gyrus section
• Species: Rabbit
• Lot: VIY0218061

reviewed by: Wei-Sheng Chen WB Human 07/13/2015

Summary

• Application: Western Blot
• Sample Tested: primary human lymphatic endothelial cells
• Species: Human

reviewed by: Verified Customer IF Mouse 01/07/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 23299940
• Species: Mouse

reviewed by: Verified Customer IHC-P Other 01/07/2015

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: See PMID 22115316
• Species: Other

Bioinformatics

• Gene Symbol: PROX1
• Uniprot:
  • Q92786()