Protein Phosphatase 1 beta Antibody

Western Blot: Protein Phosphatase 1 beta Antibody [NB110-40550] - Detection of Human PPP1CB on HeLa whole cell lysate using NB110-40550. PPP1CC was immunoprecipitated by rabbit anti-PPP1CC antibody NB110-40551.

Immunohistochemistry-Paraffin: Protein Phosphatase 1 beta Antibody [NB110-40550] - Human breast carcinoma. Antibody: Affinity purified rabbit anti- PPP1CB used at a dilution of 1:200 (1ug/ml). Detection: DAB

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Ch, Pm, Rb, Xp
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Protein Phosphatase 1 beta Antibody Summary

• Description: Novus Biologicals Rabbit Protein Phosphatase 1 beta Antibody (NB110-40550) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 280 and the C-terminus (residue 327) of human Protein Phosphatase 1, Catalytic Subunit, Beta Isoform using the numbering given in entry NP_996759.1 (GeneID 5500).
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Rabbit (100%), Chicken (100%), Canine (100%), Primate (100%), Xenopus (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PPP1CB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:250
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections
• Theoretical MW: 37 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Protein Phosphatase 1 beta Overexpression Lysate

Reactivity Notes

Orangutan (100%).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Protein Phosphatase 1 beta Antibody

• EC 3.1.3.16
• EC 3.1.3.53
• MGC3672
• PP1beta
• PP-1Bprotein phosphatase 1, catalytic subunit, delta isoform
• PPP1CD
• protein phosphatase 1, catalytic subunit, beta isoform
• protein phosphatase 1, catalytic subunit, beta isozyme
• protein phosphatase 1-beta
• protein phosphatase 1-delta
• serine/threonine protein phosphatase PP1-beta catalytic subunit
• serine/threonine-protein phosphatase PP1-beta catalytic subunit

Background

The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Mouse studies suggest that PP1 functions as a suppressor of learning and memory. Two alternatively spliced transcript variants encoding distinct isoforms have been observed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPP1CB
• Entrez:
  • 5500(Human)
• Uniprot:
  • P62140(Human)