Protein Disulfide Isomerase/P4HB Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody [NBP1-40137] - Analysis of C2C12 cells using NBP1-40137 (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin ...read more
Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP1-40137] - Staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, MkSpecies Glossary
Applications WB, ICC/IF, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Protein Disulfide Isomerase/P4HB Antibody Summary

Immunogen
Synthetic peptide derived from the sequence around Pro329 of human PDI (KLH).
Marker
Fibroblast Marker
Specificity
Recognizes endogenous levels of total human PDI protein. Species cross-reactivity: monkey, mouse and rat.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
P4HB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry-Paraffin 1:50
Control
Protein Disulfide Isomerase/P4HB Lysate (NBP2-64836)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10mM Sodium HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
50% glycerol
Purity
Immunogen affinity purified

Alternate Names for Protein Disulfide Isomerase/P4HB Antibody

  • Cellular thyroid hormone-binding protein
  • collagen prolyl 4-hydroxylase beta
  • DSI
  • ERBA2L
  • GIT
  • glutathione-insulin transhydrogenase
  • P4HB
  • P4Hbeta
  • p55
  • PDI
  • PDIA1
  • PDIA1procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide (protein disulfide isomerase-associated 1)
  • PDIEC 5.3.4.1
  • PHDB
  • PO4DB
  • PO4HB
  • procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide
  • PROHB
  • PROHBprocollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide (protein disulfide isomerase; thyroid hormone binding protein p55)
  • Prolyl 4-hydroxylase subunit beta
  • prolyl 4-hydroxylase, beta polypeptide
  • protein disulfide isomerase family A, member 1
  • protein disulfide isomerase/oxidoreductase
  • protein disulfide isomerase-associated 1
  • protein disulfide-isomerase
  • protocollagen hydroxylase
  • Thbp
  • thyroid hormone-binding protein p55

Background

Collagen prolyl 4-hydroxylases (P4Hs) play an essential role in the synthesis of all collagens. Two alpha and two beta subunits assemble into P4H tetramers in which protein disulfide isomerase (PDI) acts as the beta subunit.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol P4HB
Entrez
Uniprot