Protein Disulfide Isomerase/P4HB Antibody

Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP1-40137] - Staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.

Product Discontinued

Protein Disulfide Isomerase/P4HB Antibody Summary

• Immunogen: Synthetic peptide derived from the sequence around Pro329 of human Protein Disulfide Isomerase/P4HB (KLH).
• Epitope: Around Pro329 of human protein
• Marker: Fibroblast Marker
• Specificity: Recognizes endogenous levels of total human Protein Disulfide Isomerase/P4HB protein. Species cross-reactivity: monkey, mouse and rat.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: P4HB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 1:50
  • Immunocytochemistry/ Immunofluorescence
  • Immunofluorescence 1:50
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:1000
• Application Notes: IHC: NBP1-40137 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. Immunofluorescence (IF-IC): Requires methanol permeabilization.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10mM Sodium HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA
• Preservative: 50% glycerol
• Purity: Immunogen affinity purified

Alternate Names for Protein Disulfide Isomerase/P4HB Antibody

• Cellular thyroid hormone-binding protein
• collagen prolyl 4-hydroxylase beta
• DSI
• ERBA2L
• GIT
• glutathione-insulin transhydrogenase
• P4HB
• P4Hbeta
• p55
• PDI
• PDIA1
• PDIA1procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide (protein disulfide isomerase-associated 1)
• PDIEC 5.3.4.1
• PHDB
• PO4DB
• PO4HB
• procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide
• PROHB
• PROHBprocollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide (protein disulfide isomerase; thyroid hormone binding protein p55)
• Prolyl 4-hydroxylase subunit beta
• prolyl 4-hydroxylase, beta polypeptide
• protein disulfide isomerase family A, member 1
• protein disulfide isomerase/oxidoreductase
• protein disulfide isomerase-associated 1
• protein disulfide-isomerase
• protocollagen hydroxylase
• Thbp
• thyroid hormone-binding protein p55

Background

P4HB, the beta subunit of prolyl 4-hydroxylase, a highly abundant member of the PDI family. P4HB is a multifunctional enzyme, involved in hydroxylation of prolyl residues in preprocollagen, as well as continual rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: P4HB
• Entrez:
  • 5034(Human)
• Uniprot:
  • P07237()