Proteasome beta 1 Antibody - BSA Free

Western Blot: Proteasome beta 1 Antibody [NBP3-12968] - Proteasome beta 1 antibody detects Proteasome beta 1 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Proteasome beta 1 antibody (NBP3-12968) diluted at 1:1000.

Immunohistochemistry-Paraffin: Proteasome beta 1 Antibody [NBP3-12968] - Proteasome beta 1 antibody detects Proteasome beta 1 protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Proteasome beta 1 antibody (NBP3-12968) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: Proteasome beta 1 Antibody [NBP3-12968] - Proteasome beta 1 antibody detects Proteasome beta 1 protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Proteasome beta 1 antibody (NBP3-12968) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu, Mu, Rt, Po, Bv, RM
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Proteasome beta 1 Antibody - BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Proteasome beta 1. The exact sequence is proprietary.
• Predicted Species: Porcine (97%), Bovine (95%), Rhesus Macaque (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PSMB1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot

Reactivity Notes

Zebrafish (80%), Chicken (85%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Proteasome beta 1 Antibody - BSA Free

• EC 3.4.25.1
• FLJ25321
• HC5
• KIAA1838
• Macropain subunit C5
• Multicatalytic endopeptidase complex subunit C5
• PMSB1
• proteasome (prosome, macropain) subunit, beta type, 1
• proteasome beta 1 subunit
• Proteasome beta 1
• Proteasome component C5
• Proteasome gamma chain
• proteasome subunit beta type-1
• proteasome subunit HC5
• PSC5
• PSMB1

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is tightly linked to the TBP (TATA-binding protein) gene in human and in mouse, and is transcribed in the opposite orientation in both species. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PSMB1