Proteasome 20S beta2 Antibody

Images

 
Western Blot: Proteasome 20S beta2 Antibody [NBP1-54590] - Analysis of HepG2 cell lysate. Antibody Dilution: 1.0 ug/ml PSMB2 is supported by BioGPS gene expression data to be expressed in HepG2.
Western Blot: Proteasome 20S beta2 Antibody [NBP1-54590] - Titration: 0.2-1 ug/ml, Positive Control: Hela cell lysate.
Western Blot: Proteasome 20S beta2 Antibody [NBP1-54590] - Jurkat, Antibody Dilution: 1.0 ug/ml PSMB2 is strongly supported by BioGPS gene expression data to be expressed in Jurkat.
Western Blot: Proteasome 20S beta2 Antibody [NBP1-54590] - Titration: 2 ug/ml Positive Control: Human NT-2 cells and Mouse WT brain.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Proteasome 20S beta2 Antibody Summary

Immunogen
Synthetic peptides corresponding to PSMB2(proteasome (prosome, macropain) subunit, beta type, 2) The peptide sequence was selected from the middle region of PSMB2 (NP_002785). Peptide sequence LDRYYTPTISRERAVELLRKCLEELQKRFILNLPTFSVRIIDKNGIHDLD.
Specificity
This product is specific to Subunit or Isoform: beta type-2.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PSMB2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.2 ug/ml
Application Notes
This is a rabbit polyclonal antibody against PSMB2 and was validated on Western blot.
Theoretical MW
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 2% Sucrose
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for Proteasome 20S beta2 Antibody

  • EC 3.4.25.1
  • HC7-I
  • Macropain subunit C7-I
  • MGC104215
  • MGC126885
  • multicatalytic endopeptidase complex subunit C7-1
  • Multicatalytic endopeptidase complex subunit C7-I
  • proteasome (prosome, macropain) subunit, beta type, 2
  • proteasome beta 2 subunit
  • Proteasome component C7-I
  • proteasome subunit beta type-2
  • proteasome subunit, beta type, 2

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMB2 is a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PSMB2
Entrez
Uniprot