PSMB7 Antibody (OTI1E1)

Images

 
Western Blot: PSMB7 Antibody (OTI1E1) [NBP2-01832] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Proteasome 20S beta 7 (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Immunohistochemistry-Paraffin: PSMB7 Antibody (OTI1E1) [NBP2-01832] - Staining of paraffin-embedded Human prostate tissue using anti-Proteasome 20S beta 7 mouse monoclonal antibody.
Western Blot: PSMB7 Antibody (OTI1E1) [NBP2-01832] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Proteasome 20S beta 7 monoclonal antibody.
Immunohistochemistry-Paraffin: PSMB7 Antibody (OTI1E1) [NBP2-01832] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-Proteasome 20S beta 7 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PSMB7 Antibody (OTI1E1) [NBP2-01832] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Proteasome 20S beta 7 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PSMB7 Antibody (OTI1E1) [NBP2-01832] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Proteasome 20S beta 7 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PSMB7 Antibody (OTI1E1) [NBP2-01832] - Staining of paraffin-embedded Human breast tissue using anti-Proteasome 20S beta 7 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PSMB7 Antibody (OTI1E1) [NBP2-01832] - Staining of paraffin-embedded Human colon tissue using anti-Proteasome 20S beta 7 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, PmSpecies Glossary
Applications WB, IHC, IHC-P
Clone
OTI1E1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

PSMB7 Antibody (OTI1E1) Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 58-277 of human PSMB7(NP_002790) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PSMB7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
Theoretical MW
29.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP2-01832.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PSMB7 Antibody (OTI1E1)

  • Macropain Chain Z
  • Proteasome Subunit Z
  • PSMB7
  • Z

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. Expression of this catalytic subunit is downregulated by gamma interferon and proteolytic processing is required to generate a mature subunit. This subunit is not present in the immunoproteasome and is replaced by catalytic subunit 2i (proteasome beta 10 subunit). [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PSMB7 Antibody (NBP2-01832)(1)


Showing Publication 1 - 1 of 1.
Publication using NBP2-01832 Applications Species
Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PSMB7
Entrez