Proteasome 20S alpha 5 Antibody

Images

 
Western Blot: Proteasome 20S alpha 5 Antibody [NBP1-32387] - Sample(30 ug whole cell lysate)A: HeLa S3 12% SDS PAGE, antibody diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: Proteasome 20S alpha 5 Antibody [NBP1-32387] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: Proteasome 20S alpha 5 Antibody [NBP1-32387] - Paraffin-embedded H1299 xenograft, using antibody at 1:100 dilution.
Western Blot: Proteasome 20S alpha 5 Antibody [NBP1-32387] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE; antibody diluted at 1:5000.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.96 mg/ml

Order Details

Proteasome 20S alpha 5 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human PSMA5. The exact sequence is proprietary.
Localization
Cytoplasm, Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PSMA5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:5000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000

Reactivity Notes

Expected cross reactivity based on sequence homology: Rhesus Monkey (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.96 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Proteasome 20S alpha 5 Antibody

  • EC 3.4.25.1
  • FLJ42315
  • macropain subunit zeta
  • Macropain zeta chain
  • MGC117302
  • MGC125802
  • MGC125803
  • MGC125804
  • Multicatalytic endopeptidase complex zeta chain
  • proteasome (prosome, macropain) subunit, alpha type, 5
  • proteasome alpha 5 subunit
  • proteasome component 5
  • proteasome subunit alpha type-5
  • proteasome subunit zeta
  • Proteasome zeta chain
  • PSC5
  • ZETA

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PSMA5