Proteasome 19S 10B Antibody (OTI1G6) - Azide and BSA Free

Western Blot: Proteasome 19S 10B Antibody (OTI1G6) - Azide and BSA Free [NBP2-73627] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Proteasome 19S 10B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Pro

Immunocytochemistry/Immunofluorescence: Proteasome 19S 10B Antibody (OTI1G6) - Azide and BSA Free [NBP2-73627] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Proteasome 19S 10B.

Immunohistochemistry: Proteasome 19S 10B Antibody (OTI1G6) - Azide and BSA Free [NBP2-73627] - Staining of paraffin-embedded Human Kidney tissue using anti-Proteasome 19S 10B mouse monoclonal antibody.

Western Blot: Proteasome 19S 10B Antibody (OTI1G6) - Azide and BSA Free [NBP2-73627] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Proteasome 19S 10B monoclonal antibody.

Immunohistochemistry: Proteasome 19S 10B Antibody (OTI1G6) - Azide and BSA Free [NBP2-73627] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Proteasome 19S 10B mouse monoclonal antibody.

Immunohistochemistry: Proteasome 19S 10B Antibody (OTI1G6) - Azide and BSA Free [NBP2-73627] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Proteasome 19S 10B mouse monoclonal antibody.

Immunohistochemistry: Proteasome 19S 10B Antibody (OTI1G6) - Azide and BSA Free [NBP2-73627] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Proteasome 19S 10B mouse monoclonal antibody.

Immunohistochemistry: Proteasome 19S 10B Antibody (OTI1G6) - Azide and BSA Free [NBP2-73627] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Proteasome 19S 10B mouse monoclonal antibody.

Immunohistochemistry: Proteasome 19S 10B Antibody (OTI1G6) - Azide and BSA Free [NBP2-73627] - Staining of paraffin-embedded Human breast tissue using anti-Proteasome 19S 10B mouse monoclonal antibody.

Immunohistochemistry: Proteasome 19S 10B Antibody (OTI1G6) - Azide and BSA Free [NBP2-73627] - Staining of paraffin-embedded Human pancreas tissue using anti-Proteasome 19S 10B mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt, Ca, Pm, Pm
• Applications: WB, ICC/IF, IHC
• Clone: OTI1G6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Proteasome 19S 10B Antibody (OTI1G6) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Proteasome 19S 10B Antibody (OTI1G6) - Azide and BSA Free (NBP2-00990) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human PSMD2(NP_002799) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: PSMD2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-2000
• Theoretical MW: 100 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Proteasome 19S 10B Antibody (OTI1G6) - Azide and BSA Free

• 26S proteasome regulatory subunit RPN1
• 26S proteasome regulatory subunit S2
• MGC14274,55.11 protein
• P97
• proteasome (prosome, macropain) 26S subunit, non-ATPase, 2
• Protein 55.11
• Rpn1
• S226S proteasome non-ATPase regulatory subunit 2
• TNFR-associated protein 2
• TRAP226S proteasome subunit p97
• tumor necrosis factor receptor-associated protein 2
• Tumor necrosis factor type 1 receptor-associated protein 2

Background

Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Others are degraded via the vacuolar pathway which includes endosomes, lysosomes and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multi-subunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis, Angelman's syndrome and Liddle syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PSMD2