Proteasome 19S 10B Antibody (OTI1G6)

Western Blot: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Proteasome 19S 10B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Proteasome 19S 10B.

Immunocytochemistry/Immunofluorescence: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Proteasome 19S 10B.

Immunohistochemistry-Paraffin: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - Staining of paraffin-embedded Human pancreas tissue using anti-Proteasome 19S 10B mouse monoclonal antibody.

Western Blot: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] Analysis of extracts (35ug) from 9 different cell lines by using anti-Proteasome 19S 10B monoclonal antibody.

Immunohistochemistry-Paraffin: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Proteasome 19S 10B mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Proteasome 19S 10B mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Proteasome 19S 10B mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Proteasome 19S 10B mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - Staining of paraffin-embedded Human breast tissue using anti-Proteasome 19S 10B mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - Staining of paraffin-embedded Human Kidney tissue using anti-Proteasome 19S 10B mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt, Ca, Mk, Pm
• Applications: WB, ICC/IF, IHC, IHC-P
• Clone: OTI1G6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Proteasome 19S 10B Antibody (OTI1G6) Summary

• Immunogen: Full length human recombinant protein of human PSMD2(NP_002799) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: PSMD2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-2000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Proteasome 19S 10B Antibody (OTI1G6)

• 26S proteasome regulatory subunit RPN1
• 26S proteasome regulatory subunit S2
• MGC14274,55.11 protein
• P97
• proteasome (prosome, macropain) 26S subunit, non-ATPase, 2
• Protein 55.11
• Rpn1
• S226S proteasome non-ATPase regulatory subunit 2
• TNFR-associated protein 2
• TRAP226S proteasome subunit p97
• tumor necrosis factor receptor-associated protein 2
• Tumor necrosis factor type 1 receptor-associated protein 2

Background

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. In addition to participation in proteasome function, this subunit may also participate in the TNF signalling pathway since it interacts with the tumor necrosis factor type 1 receptor. A pseudogene has been identified on chromosome 1. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PSMD2
• Entrez:
  • 5708(Human)