Proteasome 19S 10B Antibody (OTI1G6)

Images

 
Western Blot: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Proteasome 19S 10B (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Immunocytochemistry/ Immunofluorescence: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Proteasome 19S 10B.
Immunohistochemistry-Paraffin: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - Staining of paraffin-embedded Human pancreas tissue using anti-Proteasome 19S 10B mouse monoclonal antibody.
Western Blot: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] Analysis of extracts (35ug) from 9 different cell lines by using anti-Proteasome 19S 10B monoclonal antibody.
Immunohistochemistry-Paraffin: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Proteasome 19S 10B mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Proteasome 19S 10B mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Proteasome 19S 10B mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Proteasome 19S 10B mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - Staining of paraffin-embedded Human breast tissue using anti-Proteasome 19S 10B mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - Staining of paraffin-embedded Human Kidney tissue using anti-Proteasome 19S 10B mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Pm, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
OTI1G6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.7 mg/ml

Proteasome 19S 10B Antibody (OTI1G6) Summary

Immunogen
Full length human recombinant protein of human PSMD2(NP_002799) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
PSMD2
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150
Theoretical MW
100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.7 mg/ml
Purity
Affinity purified

Alternate Names for Proteasome 19S 10B Antibody (OTI1G6)

  • 26S proteasome regulatory subunit RPN1
  • 26S proteasome regulatory subunit S2
  • MGC14274,55.11 protein
  • P97
  • proteasome (prosome, macropain) 26S subunit, non-ATPase, 2
  • Protein 55.11
  • Rpn1
  • S226S proteasome non-ATPase regulatory subunit 2
  • TNFR-associated protein 2
  • TRAP226S proteasome subunit p97
  • tumor necrosis factor receptor-associated protein 2
  • Tumor necrosis factor type 1 receptor-associated protein 2

Background

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. In addition to participation in proteasome function, this subunit may also participate in the TNF signalling pathway since it interacts with the tumor necrosis factor type 1 receptor. A pseudogene has been identified on chromosome 1. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-73627AF350
Alexa Fluor 405 NBP2-73627AF405
Alexa Fluor 488 NBP2-73627AF488
Alexa Fluor 532 NBP2-73627AF532
Alexa Fluor 594 NBP2-73627AF594
Alexa Fluor 647 NBP2-73627AF647
Alexa Fluor 700 NBP2-73627AF700
Alexa Fluor 750 NBP2-73627AF750
Biotin NBP2-73627B
DyLight 350 NBP2-73627UV
DyLight 405 NBP2-73627V
DyLight 488 NBP2-73627G
DyLight 550 NBP2-73627R
DyLight 594 NBP2-73627DL594
DyLight 650 NBP2-73627C
DyLight 680 NBP2-73627FR
DyLight 755 NBP2-73627IR
FITC NBP2-73627F
HRP NBP2-73627H
Janelia Fluor 549 NBP2-73627JF549
Janelia Fluor 646 NBP2-73627JF646

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Bioinformatics

Gene Symbol PSMD2
Entrez