Prostatic Acid Phosphatase/ACPP Antibody (690017) [Alexa Fluor® 350]

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 690017
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 350

Prostatic Acid Phosphatase/ACPP Antibody (690017) [Alexa Fluor® 350] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Prostatic Acid Phosphatase/ACPP
  Lys33-Gln379
  Accession # P15309
• Specificity: Detects human Prostatic Acid Phosphatase/ACPP in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human TRACP/ACP5 is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Prostatic Acid Phosphatase/ACPP Antibody (690017) [Alexa Fluor® 350]

• acid phosphatase, prostate
• ACP3
• ACP-3
• ACPP
• EC 3.1.3.2
• PAP
• Prostatic Acid Phosphatase
• prostatic acid phosphotase

Background

ACPP (Acid phosphatase, prostate; also PAP and ACP3) is an approximately 50 kDa glycoprotein member of the histidine acid phosphatase family of enzymes. It exists as a 95‑100 kDa nondisulfide-linked homodimer that hydrolyzes phosphate esters under low pH to generate free phosphate. ACPP is expressed by prostate epithelium and pain-detecting spinal cord neurons. In the spinal cord, ACPP dephosphorylates AMP. This generates adenosine which acts as a strong analgesic agent. Mature human ACPP is 354 amino acids (aa) in length (aa 33-386). It contains one histidine phosphatase domain (aa 34-332), plus a nucleophile acceptor site at His44, and a proton donor site at Asp290. There are two potential alternative splice variants. One shows a deletion of aa 153-185, while another is transmembrane (previously called TMPase) and shows a 38 aa substitution for the C-terminal seven amino acids. Over aa 33-379, human ACPP shares 84% aa identity with mouse ACPP.          

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.