Prolactin R Antibody (U5) [Allophycocyanin]

• Reactivity: Hu, Mu, Rt, Po, Bv, Eq, Ha, Rb, Sh
• Applications: WB, Flow, ICC/IF, IHC, IP
• Clone: U5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

Prolactin R Antibody (U5) [Allophycocyanin] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: Purified rat liver PRL receptor.
• Epitope: This antibody has been shown to react with the extracellular portion of the receptor, but not in the ligand binding domain.
• Localization: Cytoplasmic
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PRLR
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NB300-561. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Reactivity Notes

Sheep reactivity reported in scientific literature (PMID: 2707162). Bovine reactivity reported in scientific literature (PMID: 25212395). Mouse reactivity reported in scientific literature (PMID: 19211889). Hamster reactivity reported in scientific literature (PMID: 24142695). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for Prolactin R Antibody (U5) [Allophycocyanin]

• delta 4-delta 7/11 truncated prolactin receptor
• delta 4-SF1b truncated prolactin receptor
• hPRL receptor
• hPRLrI
• PRLR
• PRL-R
• Prolactin R
• prolactin receptor delta 7/11
• prolactin receptor
• ProlactinR
• secreted prolactin binding protein

Background

Prolactin (PRL) is a hormone involved in a variety of important functions including ion transport and osmoregulation, stimulation of milk, protein synthesis as well as the regulation of numerous reproductive functions. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor. Immunoreactive PRL receptor, a member of the cytokine receptor family, varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa. The PRL receptor consists of at least three separate domains: an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Prolactin R Antibody (NB300-561APC)(1)

Publication using NB300-561APC

• Ochoa-Amaya JE, Queiroz-Hazarbassanov N, Namazu LB et al. Short-Term Hyperprolactinemia Reduces the Expression of Purinergic P2X7 Receptors during Allergic Inflammatory Response of the Lungs Neuroimmunomodulation 2018 Jun 06 [PMID: 29874677] (ICC/IF, Rat) Neuroimmunomodulation. [PMID: 29874677] (ICC/IF, Rat)

Bioinformatics

• Gene Symbol: PRLR
• Entrez:
  • 5618(Human)
  • 414916(Porcine)
  • 100009046(Rabbit)
  • 24684(Rat)