Human Prolactin ELISA Kit (Colorimetric)

ELISA: Human Prolactin ELISA Kit (Colorimetric) [NBP2-60128] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Human Prolactin ELISA Kit (Colorimetric) [NBP2-60128] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, cell culture supernates, tissue homogenates and cell lysates
• Standard Curve Range: 40 uIU/mL - 2000 uIU/mL
• Sensitivity: 50 uIU/mL

Human Prolactin ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 1-5 hours
• Specificity: This assay has high sensitivity and excellent specificity for detection of human Prolactin. No significant cross-reactivity or interference between human Prolactin and analogues was observed
• Standard Curve Range: 40 uIU/mL - 2000 uIU/mL
• Sensitivity: 50 uIU/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 15
• Intra-Assay: %CV < 15
• Sample Volume: 50 - 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: PRL

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Prolactin ELISA Kit (Colorimetric)

• PRL
• Prolactin

Background

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PRL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for PRL. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRL bound in the initial step. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Prolactin ELISA Kit (NBP2-60128)(1)

Publication using NBP2-60128

• Palomar A, Quiñonero A, Medina-Laver Y et al. Antioxidant Supplementation Alleviates Mercury-Induced Cytotoxicity and Restores the Implantation-Related Functions of Primary Human Endometrial Cells International journal of molecular sciences 2023-05-15 [PMID: 37240143] (ELISA)

Bioinformatics

• Gene Symbol: PRL