ELISA: Human Prolactin ELISA Kit (Colorimetric) [NBP2-60128] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Human Prolactin ELISA Kit (Colorimetric) [NBP2-60128] - Standard Curve Reference
Product Details
Summary
Reactivity
HuSpecies Glossary
Applications
ELISA
Suitable Sample Type
Serum, plasma, cell culture supernates, tissue homogenates and cell lysates
This assay has high sensitivity and excellent specificity for detection of human Prolactin. No significant cross-reactivity or interference between human Prolactin and analogues was observed
Storage of components varies. See protocol for specific instructions.
Kit Components
Components
Adhesive Strip (For 96 wells)
Assay plate
HRP-conjugate
Instruction manual
Standard
Stop Solution
Substrate A
Substrate B
Wash Buffer (20 x concentrate)
Alternate Names for Human Prolactin ELISA Kit (Colorimetric)
PRL
Prolactin
Background
This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PRL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for PRL. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRL bound in the initial step. The color development is stopped and the intensity of the color is measured.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.