Prolactin Antibody (OTI6B1)

Western Blot: Prolactin Antibody (6B1) [NBP2-02142] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Prolactin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Prolactin.

Immunocytochemistry/Immunofluorescence: Prolactin Antibody (6B1) [NBP2-02142] Staining of COS7 cells transiently transfected by pCMV6-ENTRY Prolactin.

Immunohistochemistry-Paraffin: Prolactin Antibody (6B1) [NBP2-02142] - Staining of paraffin-embedded Human Ovary tissue using anti-Prolactin mouse monoclonal antibody.

Flow Cytometry: Prolactin Antibody (6B1) [NBP2-02142] - Analysis of Jurkat cells, using anti-Prolactin antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Immunocytochemistry/Immunofluorescence: Prolactin Antibody (6B1) [NBP2-02142] - Immunofluorescent staining of HepG2 cells using anti-Prolactin mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Prolactin Antibody (6B1) [NBP2-02142] - Staining of paraffin-embedded Human breast tissue using anti-Prolactin mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Prolactin Antibody (6B1) [NBP2-02142] - Staining of paraffin-embedded Human endometrium tissue using anti-Prolactin mouse monoclonal antibody.

Flow Cytometry: Prolactin Antibody (6B1) [NBP2-02142] - Analysis of Hela cells, using anti-Prolactin antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI6B1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Prolactin Antibody (OTI6B1) Summary

• Description: Novus Biologicals Mouse Prolactin Antibody (OTI6B1) (NBP2-02142) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. Anti-Prolactin Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human PRL (NP_000939) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: PRL
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:1000
• Theoretical MW: 25.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Prolactin Antibody (OTI6B1)

• PRL
• Prolactin

Background

Various hormones are secreted from the anterior pituitary during development and growth. Prolactin is a growth factor secreted by the anterior pituitary that is necessary for the proliferation and differentiation of the mammary glands.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Prolactin Antibody (NBP2-02142)(1)

Publication using NBP2-02142

• J�ngert K, Paulsen F, Jacobi C et al. Prolactin Inducible Protein, but Not Prolactin, Is Present in Human Tears, Is Involved in Tear Film Quality, and Influences Evaporative Dry Eye Disease Frontiers in Medicine 2022-06-30 [PMID: 35847789] (Immunohistochemistry, Immunohistochemistry-Paraffin)

Bioinformatics

• Gene Symbol: PRL
• Entrez:
  • 5617(Human)