Prohibitin Antibody (II-14-10) - IHC-Prediluted

Images

 
Immunohistochemistry-Paraffin: Prohibitin Antibody (II-14-10) [NB110-59934] - Staining of liver hepato cellular carcinoma.
Immunohistochemistry: Prohibitin Antibody (II-14-10) [NB110-59934] - Formalin fixed paraffin embedded human heart stained with Prohibitin antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-Fr, IHC-P
Clone
II-14-10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
IHC-Prediluted

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Catalog# & Formulation Size Price

Prohibitin Antibody (II-14-10) - IHC-Prediluted Summary

Immunogen
BALB/C mice were injected with purified recombinant rat prohibitin protein.
Localization
Cytoplasmic
Marker
Mitochondria Marker
Specificity
This is specific to 30kD protein known as Prohibitin. Prohibitin is an evolutionarily conserved protein located in the innermembrane of mitochondria. Prohibitin shows antiproliferative activity and has been proposed to play a role in normal cell cycle regulation, replicative senescence, cellular immortalization, and tumor suppression. This antibody cross reacts with mouse, rat, and pig.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PHB
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen Neat
  • Immunohistochemistry-Paraffin Neat
Application Notes
This antibody works in, Western Blot, Immunohistochemistry-Paraffin and Immunohistochemistry-Frozen. Although not tested this antibody may be useful in Immunofluorescence/Immunocytochemistry.
Positive Control
Heart Lysate (NB820-59217)
Publications
Read Publications using
NB110-59934 in the following applications:

  • WB
    3 publications

Reactivity Notes

This antibody is reactive against pig. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
1.0% BSA
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

This product has a prediluted size available. If a 6mL or 7mL pre-diluted size is purchased this product can be used Neat (without further dilution). Use recommended dilution for the concentrated vials only.

Alternate Names for Prohibitin Antibody (II-14-10) - IHC-Prediluted

  • PHB
  • PHB1
  • Prohibitin

Background

Prohibitin is an evolutionarily conserved protein located in the inner membrane of mitochondria. Prohibitin shows antiproliferative activity and has been proposed to play a role in normal cell cycle regulation, replicative senescence, cellular immortalization and tumor suppression. Prohibitin is also sometimes found in the nucleus in certain cells types and cell states.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Prohibitin Antibody (NB110-59934)(3)

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Positive Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol PHB
Entrez