Prohibitin Antibody (II-14-10) - IHC-Prediluted

Immunohistochemistry-Paraffin: Prohibitin Antibody (II-14-10) - IHC-Prediluted [NB110-59934] - Formalin fixed paraffin embedded liver hepato cellular carcinoma stained with Prohibitin antibody (NB110-59934).

Product Discontinued

Prohibitin Antibody (II-14-10) - IHC-Prediluted Summary

• Immunogen: BALB/C mice were injected with purified recombinant rat prohibitin protein.
• Localization: Cytoplasmic
• Marker: Mitochondria Marker
• Specificity: This is specific to 30kD protein known as Prohibitin. Prohibitin is an evolutionarily conserved protein located in the innermembrane of mitochondria. Prohibitin shows antiproliferative activity and has been proposed to play a role in normal cell cycle regulation, replicative senescence, cellular immortalization, and tumor suppression. This antibody cross reacts with mouse, rat, and pig.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PHB1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Frozen Neat
  • Immunohistochemistry-Paraffin Neat
  • Western Blot
• Application Notes: This antibody works in , Western Blot, Immunohistochemistry-Paraffin and Immunohistochemistry-Frozen. Although not tested this antibody may be useful in Immunofluorescence/Immunocytochemistry. Use in Western Blot reported in scientific literature (PMID:22904309).
• Control:

  Human Heart Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

This antibody is reactive against pig. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 1.0% BSA
• Preservative: 0.09% Sodium Azide
• Purity: Protein A or G purified

Notes

This product has a prediluted size available. If a 6mL or 7mL pre-diluted size is purchased this product can be used Neat (without further dilution). Use recommended dilution for the concentrated vials only.

Alternate Names for Prohibitin Antibody (II-14-10) - IHC-Prediluted

• PHB
• PHB1
• Prohibitin

Background

Prohibitin is an evolutionarily conserved protein located in the inner membrane of mitochondria. Prohibitin shows antiproliferative activity and has been proposed to play a role in normal cell cycle regulation, replicative senescence, cellular immortalization and tumor suppression. Prohibitin is also sometimes found in the nucleus in certain cells types and cell states.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Prohibitin Antibody (NB110-59934)(3)

Publications using NB110-59934

• Lugini L, Cecchetti S, Huber V et al. Immune surveillance properties of human NK cell-derived exosomes. J Immunol. [PMID: 22904309] (WB, Human)
  
  Details:
  Citation using the IHC-Prediluted form of this antibody.
• Pellegrini L, Pucci B, Villanova L et al. SIRT3 protects from hypoxia and staurosporine-mediated cell death by maintaining mitochondrial membrane potential and intracellular pH Cell Death Differ [PMID: 22595756] (WB, Human)
  
  Details:
  Used the IHC-Prediluted form of this antibody.
• Xu Z, Wu J, Zha X. Up-regulation of prohibitin 1 is involved in the proliferation and migration of liver cancer cells. Sci China Life Sci. [PMID: 21318481] (WB, Human)
  
  Details:
  Citation using the IHC-Prediluted form of this antibody.

Bioinformatics

• Gene Symbol: PHB1
• Entrez:
  • 5245(Human)
  • 18673(Mouse)
  • 25344(Rat)