PRKCDBP Antibody (8D3) - Azide and BSA Free

Western Blot: PRKCDBP Antibody (8D3) [H00112464-M04] - Analysis of PRKCDBP expression in transfected 293T cell line by PRKCDBP monoclonal antibody (M04), clone 8D3. Lane 1: PRKCDBP transfected lysate (Predicted MW: 27.6 KDa). Lane 2: Non-transfected lysate.

Sandwich ELISA: PRKCDBP Antibody (8D3) [H00112464-M04] - Detection limit for recombinant GST tagged PRKCDBP is 0.3 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 8D3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PRKCDBP Antibody (8D3) - Azide and BSA Free Summary

• Immunogen: PRKCDBP (NP_659477, 161 a.a. ~ 261 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EVGESSDEEPVESRAQRLRRTGLQKVQSLRRALSGRKGPAAPPPTPVKPPRLGPGRSAEAQPEAQPALEPTLEPEPPQDTEEDPGRPGAAEEALLQMESVA
• Specificity: This product is specific for Human PRKCDBP monoclonal antibody (M04), clone 8D3 [Gene ID: 112464].
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CAVIN3
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot
• Application Notes: Mouse monoclonal antibody raised against a partial recombinant PRKCDBP. Use in ICC/IF was reported in scientific literature (PMID: 31332168).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PRKCDBP Antibody (8D3) - Azide and BSA Free

• Cavin-3
• HSRBC
• MGC20400
• protein kinase C, delta binding protein
• sdr-related gene product that binds to c-kinase
• Serum deprivation response factor-related gene product that binds to C-kinase
• SRBCprotein kinase C delta-binding protein

Background

PRKCDBP, also known as Protein kinase C delta-binding protein, is 261 amino acids in length and approximately 27kDa. PRKCDBP is highly expressed in epithelial cells, and is down regulated in lung cancer cell lines and breast cancer cell lines, thus suggesting the possibility of tumor suppressor function. PRKCDBP binds and is phosphorylated by protein kinase C, delta. Current research surrounding PRKCDBP shows a possible connection with ovarian cancer, gastric cancer and lung cancer. PRKCDBP has also been shown to interact with Caveolin 1, Caveolin 3, cMyc, SDPR, and EPS15L1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PRKCDBP Antibody (H00112464-M04)(2)

Publications using H00112464-M04

• McMahon KA, Stroud DA, Gambin Y et al. Cavin3 released from caveolae interacts with BRCA1 to regulate the cellular stress response eLife 2021-06-18 [PMID: 34142659]
• McMahon KA, Wu Y, Gambin Y et al. Identification of intracellular cavin target proteins reveals cavin-PP1alpha interactions regulate apoptosis Nat Commun 2019-07-22 [PMID: 31332168] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: CAVIN3
• Uniprot:
  • NP_659477(Human)